CAJ | 학술논문

目的：观察中枢神经系统感染（CNSI）者在急性期与慢性期脑脊液和血清中干扰素γ（IFN-γ）、白细胞介素-10（IL-10）和S-100B蛋白含量与健康人的差异，以探讨IFN-γ、IL-10和S-100B蛋白检测的临床价值。方法回顾性分析2009年2月至2013年8月于本院住院治疗的病毒性（观察1组）和化脓性（观察2组）脑膜脑炎患者，分别为92例和68例，并选择健康体检者30例作为对照组；检测并记录观察1组和观察2组急性期（A期）、慢性期（C期）脑脊液（CSF）和血清中IFN-γ、IL-10、S-100B蛋白的含量，并与对照组相比较。结果观察1组和观察2组A期CSF和血清中IFN-γ、IL-10和S-100B蛋白含量均显著高于C期患者及对照组；观察1组A期CSF和血清中干扰素γ含量高于观察2组A期，而IL-10、S-100B蛋白含量低于观察2组A期；观察1组、观察2组A期CSF S-100B蛋白含量高于血清，而CSF IFN-γ、IL-10含量低于血清；以上各组两两比较，差异均有统计学意义（P均＜0.05）。A期CSF和血清中IFN-γ/IL-10相比较，观察1组＞对照组＞观察2组，各组差异均具有统计学意义（P均＜0.05）。结论 IFN-γ、IL-10和S-100B蛋白在发生CNSI的患者CSF和血清中含量会有显著变化，对CNSI具有重要的诊断价值，且对于病毒性脑膜脑炎和化脓性脑膜脑炎的鉴别诊断具有重要意义。
목적：관찰중추신경계통감염（CNSI）자재급성기여만성기뇌척액화혈청중간우소γ（IFN-γ）、백세포개소-10（IL-10）화S-100B단백함량여건강인적차이，이탐토IFN-γ、IL-10화S-100B단백검측적림상개치。방법회고성분석2009년2월지2013년8월우본원주원치료적병독성（관찰1조）화화농성（관찰2조）뇌막뇌염환자，분별위92례화68례，병선택건강체검자30례작위대조조；검측병기록관찰1조화관찰2조급성기（A기）、만성기（C기）뇌척액（CSF）화혈청중IFN-γ、IL-10、S-100B단백적함량，병여대조조상비교。결과관찰1조화관찰2조A기CSF화혈청중IFN-γ、IL-10화S-100B단백함량균현저고우C기환자급대조조；관찰1조A기CSF화혈청중간우소γ함량고우관찰2조A기，이IL-10、S-100B단백함량저우관찰2조A기；관찰1조、관찰2조A기CSF S-100B단백함량고우혈청，이CSF IFN-γ、IL-10함량저우혈청；이상각조량량비교，차이균유통계학의의（P균＜0.05）。A기CSF화혈청중IFN-γ/IL-10상비교，관찰1조＞대조조＞관찰2조，각조차이균구유통계학의의（P균＜0.05）。결론 IFN-γ、IL-10화S-100B단백재발생CNSI적환자CSF화혈청중함량회유현저변화，대CNSI구유중요적진단개치，차대우병독성뇌막뇌염화화농성뇌막뇌염적감별진단구유중요의의。
Objective To investigate the change of interferon-γ(IFN-γ), interleukin-10 (IL-10) and S-100B protein in cerebrospinal lfuid (CSF) and serum in acute and chronic phase of patients with central nervous system infection, and explore it’s clinical diagnostic value. Methods The clinical data of 160 cases with central nervous system infection from February 2009 to August 2013 in our hospital were analyzed, retrospectively. There were 92 cases with viral meningoencephalitis were collected as observation group 1, and 68 cases with purulent meningoencephalitis were as observation group 2, while 30 healthy people were collected as the control group. The levels of IFN-γ, IL-10 and S-100B proteins in CSF and in serum were detected and compared at acute phase (A phase) and chronic phase (C phase) of patients with central nervous system infection, respectively. Results In the groups of observation 1 and observation group 2, the levels of IFN-γ, IL-10 and S-100B proteins in CSF and serum at A phase were all higher than that in C phase and the control group. In the groups of observation 1, CSF and serum IFN-γat A phase were all higher than that in observation group 2, but the level of IL-10 and S-100B in CSF and serum were all lower than that in observation group 2. At A phase, the CSF S-100B of observation 1 and observation group 2 were all higher than that in the serum. The differences above all were with signiifcant differences (P all<0.05). The comparion of IFN-γ/IL-10 at A phase in CSF and serum showed that observation group 1>control group>observation group 2, all with signiifcant differences (P<0.05). Conclusions IFN-γ, IL-10 and S-100B protein changed signiifcantly in the CSF and serum in patients with central nervous system infection, they all have important differential diagnostic value for viral meningitis and purulent meningoencephalitis.