中国中西医结合急救杂志
中國中西醫結閤急救雜誌
중국중서의결합급구잡지
INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE IN PRACTICE OF CRITICAL CARE MEDICINE
2014年
6期
405-407
,共3页
补肾活血法%顺铂%肾损伤,急性%BALB/c小鼠%中医药治疗
補腎活血法%順鉑%腎損傷,急性%BALB/c小鼠%中醫藥治療
보신활혈법%순박%신손상,급성%BALB/c소서%중의약치료
Nourishing kidney and activating blood recipe%Cisplatin%Acute kidney injury%BALB/c mouse%Chinese medicinal therapy
目的：观察补肾活血法组方对顺铂诱导的急性肾损伤（AKI）小鼠的治疗作用。方法选择雄性BALB/c小鼠21只，按随机数字表法分为对照组、AKI模型组、补肾活血法组方治疗组，每组7只。采用单次腹腔注射顺铂20 mg/kg的方法复制AKI模型；治疗组给予补肾活血法组方10μL · g-1· d-1，AKI模型组和对照组给予等体积生理盐水灌胃；各组均连续给药6d。观察各组小鼠体质量的变化；收集各组小鼠静脉血，检测血肌酐（SCr）水平；取肾组织观察肾质量，并于光镜下观察肾组织病理学变化，计算急性肾小管坏死评分（ATN）。结果与对照组比较，AKI模型组体质量、肾质量显著降低〔体质量（g）：17.18±0.29比19.33±1.43，肾质量（g）：0.28±0.01比0.32±0.11，均P＜0.01〕，SCr、ATN评分均显著升高〔SCr（μmol/L）：86.77±10.97比14.37±0.81，ATN评分（分）：3.33±0.52比0.17±0.41，均P＜0.01〕；与AKI模型组比较，治疗组体质量（g：18.70±0.28）、肾质量（g：0.31±0.01）显著升高，SCr（μmol/L：21.98±5.52）、ATN评分（分：2.00±0.63）显著下降（均P＜0.01）。光镜下可见：AKI模型组小鼠肾小管上皮细胞水肿、坏死，肾间质有炎性细胞浸润，肾小球形态大致正常；治疗组小鼠肾小管坏死程度较AKI模型组明显减轻，肾小球大致正常。结论补肾活血法组方能降低顺铂诱导的AKI模型小鼠的SCr，改善其肾组织的病理学改变，对AKI有治疗作用。
目的：觀察補腎活血法組方對順鉑誘導的急性腎損傷（AKI）小鼠的治療作用。方法選擇雄性BALB/c小鼠21隻，按隨機數字錶法分為對照組、AKI模型組、補腎活血法組方治療組，每組7隻。採用單次腹腔註射順鉑20 mg/kg的方法複製AKI模型；治療組給予補腎活血法組方10μL · g-1· d-1，AKI模型組和對照組給予等體積生理鹽水灌胃；各組均連續給藥6d。觀察各組小鼠體質量的變化；收集各組小鼠靜脈血，檢測血肌酐（SCr）水平；取腎組織觀察腎質量，併于光鏡下觀察腎組織病理學變化，計算急性腎小管壞死評分（ATN）。結果與對照組比較，AKI模型組體質量、腎質量顯著降低〔體質量（g）：17.18±0.29比19.33±1.43，腎質量（g）：0.28±0.01比0.32±0.11，均P＜0.01〕，SCr、ATN評分均顯著升高〔SCr（μmol/L）：86.77±10.97比14.37±0.81，ATN評分（分）：3.33±0.52比0.17±0.41，均P＜0.01〕；與AKI模型組比較，治療組體質量（g：18.70±0.28）、腎質量（g：0.31±0.01）顯著升高，SCr（μmol/L：21.98±5.52）、ATN評分（分：2.00±0.63）顯著下降（均P＜0.01）。光鏡下可見：AKI模型組小鼠腎小管上皮細胞水腫、壞死，腎間質有炎性細胞浸潤，腎小毬形態大緻正常；治療組小鼠腎小管壞死程度較AKI模型組明顯減輕，腎小毬大緻正常。結論補腎活血法組方能降低順鉑誘導的AKI模型小鼠的SCr，改善其腎組織的病理學改變，對AKI有治療作用。
목적：관찰보신활혈법조방대순박유도적급성신손상（AKI）소서적치료작용。방법선택웅성BALB/c소서21지，안수궤수자표법분위대조조、AKI모형조、보신활혈법조방치료조，매조7지。채용단차복강주사순박20 mg/kg적방법복제AKI모형；치료조급여보신활혈법조방10μL · g-1· d-1，AKI모형조화대조조급여등체적생리염수관위；각조균련속급약6d。관찰각조소서체질량적변화；수집각조소서정맥혈，검측혈기항（SCr）수평；취신조직관찰신질량，병우광경하관찰신조직병이학변화，계산급성신소관배사평분（ATN）。결과여대조조비교，AKI모형조체질량、신질량현저강저〔체질량（g）：17.18±0.29비19.33±1.43，신질량（g）：0.28±0.01비0.32±0.11，균P＜0.01〕，SCr、ATN평분균현저승고〔SCr（μmol/L）：86.77±10.97비14.37±0.81，ATN평분（분）：3.33±0.52비0.17±0.41，균P＜0.01〕；여AKI모형조비교，치료조체질량（g：18.70±0.28）、신질량（g：0.31±0.01）현저승고，SCr（μmol/L：21.98±5.52）、ATN평분（분：2.00±0.63）현저하강（균P＜0.01）。광경하가견：AKI모형조소서신소관상피세포수종、배사，신간질유염성세포침윤，신소구형태대치정상；치료조소서신소관배사정도교AKI모형조명현감경，신소구대치정상。결론보신활혈법조방능강저순박유도적AKI모형소서적SCr，개선기신조직적병이학개변，대AKI유치료작용。
Objective To investigate the therapeutic effect of traditional Chinese medicine(TCM)recipe of nourishing kidney and activating blood for treatment of cisplatin induced acute kidney injury(AKI)in mouse model. Methods Twenty-one male BALB/c mice were randomly divided into three groups:control group,AKI model group and treatment group with the above TCM recipe(each n=7). The AKI model was reproduced by a single intraperitoneal injection of cisplatin 20 mg/kg;the TCM recipe with dosage of 10μL · g-1 · d-1 was given to the treatment group,while equal volume of normal saline was given to the model and control groups by gavage,lasting for 6 days in all groups. The changes of each mouse body weight were observed;mouse venous blood serum creatinine(SCr)level was detected,changes of renal tissue weight and its histopathology were observed under light microscope,and the score of acute tubular necrosis(ATN)was calculated. Results Compared to the control group,the body and renal weight in AKI model group were significantly lowered〔body weight(g):17.18±0.29 vs. 19.33±1.43,renal weight(g):0.28±0.01 vs. 0.32±0.11,both P<0.01〕,and SCr and ATN scores were significantly increased in AKI model group〔SCr(μmol/L):86.77±10.97 vs. 14.37±0.81,ATN score:3.33±0.52 vs. 0.17±0.41,both P<0.01〕. Compared to AKI model group,the body weight(g:18.70±0.28)and renal weight(g:0.31±0.01)in the treatment group were markedly increased,and SCr(μmol/L:21.98±5.52)and ATN scores(2.00±0.63)were significantly lower than those of the AKI model group(all P<0.01). Under optical microscope,there were mouse renal tubular epithelial cell edema and necrosis,renal interstitial inflammatory cell infiltration,the glomerular form roughly normal in the AKI model group;compared with the AKI model group,in the treatment group the degree of mouse renal tubular necrosis was significantly reduced and glomerular form was basically normal. Conclusion The TCM recipe of nourishing kidney and activating blood can reduce SCr and improve the pathological changes of renal tissue in cisplatin induced AKI mouse models,thus it has therapeutic effect for treatment of AKI in mice.
