宁夏农林科技
寧夏農林科技
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NINGXIA JOURNAL OF AGRICULTURE AND FORESTRY SCIENCE AND TECHNOLOGY
2014年
9期
9-10,24
,共3页
绣球绣线菊%组织培养%培养基%快繁
繡毬繡線菊%組織培養%培養基%快繁
수구수선국%조직배양%배양기%쾌번
Spiraea blumei%Tissue culture%Culture medium%Rapid propagation
应用组织培养快繁技术，对绣球绣线菊培养基和移栽过程进行研究。筛选出适宜于绣球绣线菊各时期培养的培养基配方；初代培养基配方为：MS+6-BA1.0~1.5 mg/L，继代培养基配方为：MS+6-BA1.0～1.5 mg/L+NAA0.05 mg/L，生根培养基以1/2MS+IBA0.5 mg/L或1/2MS+NAA0.5 mg/L或1/2MS+IBA0.5 mg/L+NAA0.5 mg/L的效果最好。选用珍珠岩∶蛭石∶草炭土（腐殖土）为1∶1∶0.5或砂子∶草炭土（腐殖土）为1∶1的移栽基质，移栽效果都比较好，移栽成活率达80%以上。
應用組織培養快繁技術，對繡毬繡線菊培養基和移栽過程進行研究。篩選齣適宜于繡毬繡線菊各時期培養的培養基配方；初代培養基配方為：MS+6-BA1.0~1.5 mg/L，繼代培養基配方為：MS+6-BA1.0～1.5 mg/L+NAA0.05 mg/L，生根培養基以1/2MS+IBA0.5 mg/L或1/2MS+NAA0.5 mg/L或1/2MS+IBA0.5 mg/L+NAA0.5 mg/L的效果最好。選用珍珠巖∶蛭石∶草炭土（腐殖土）為1∶1∶0.5或砂子∶草炭土（腐殖土）為1∶1的移栽基質，移栽效果都比較好，移栽成活率達80%以上。
응용조직배양쾌번기술，대수구수선국배양기화이재과정진행연구。사선출괄의우수구수선국각시기배양적배양기배방；초대배양기배방위：MS+6-BA1.0~1.5 mg/L，계대배양기배방위：MS+6-BA1.0～1.5 mg/L+NAA0.05 mg/L，생근배양기이1/2MS+IBA0.5 mg/L혹1/2MS+NAA0.5 mg/L혹1/2MS+IBA0.5 mg/L+NAA0.5 mg/L적효과최호。선용진주암∶질석∶초탄토（부식토）위1∶1∶0.5혹사자∶초탄토（부식토）위1∶1적이재기질，이재효과도비교호，이재성활솔체80%이상。
The culture medium and transplanting process of spiraea blumei were studied by using tissue culture and rapid propagation technology. The culture medium formulas suitable for all periods of spiraea blumei were screened out. The initial medium formulation is MS+6-BA1.0~1.5 mg/L,the subculture medium formula is MS+6-BA1.0~1.5 mg/L+NAA0.05 mg/L, and the most effectual rooting medium is 1/2MS+IBA0.5 mg/L or 1/2MS+NAA0.5 mg/L or 1/2MS+IBA0.5 mg/L+NAA0.5 mg/L. The selected transplanting matrix with the proportion of perlite: vermiculite : turfy soil (humus) equating to 1∶1∶0.5 or the ratio of sand: turfy soil (humus) equating to 1∶1 has a good transplanting effect and the transplanting survival rate was up to over 80%.
