中国临床保健杂志
中國臨床保健雜誌
중국림상보건잡지
CHINESE JOURNAL OF CLINICAL HEALTHCARE
2014年
6期
616-619
,共4页
胰腺肿瘤%细胞凋亡%大黄素%抗肿瘤药%小鼠,裸
胰腺腫瘤%細胞凋亡%大黃素%抗腫瘤藥%小鼠,裸
이선종류%세포조망%대황소%항종류약%소서,라
Pancreatic neoplasms%Apoptosis%Emodin%Antineoplastic agents%Mice,nude
目的：探讨大黄素增强吉西他滨对胰腺癌细胞 Bxpc-3裸鼠移植瘤的促凋亡作用及其可能的作用机制。方法在培养人胰腺癌 Bxpc-3细胞的基础上建立荷瘤模型，荷瘤裸鼠用随机区组设计分配方法分为四组：0．9％氯化钠注射溶液组（N 组）：腹腔注射0．9％氯化钠注射溶液0．2 mL，大 黄 素 组（E 组，40 mg／kg），吉西他滨组（G 组，125 mg／kg），联合组（E ＋G 组，大黄素40 mg／kg 和吉西他滨80 mg／kg）。用药均采用腹腔注射。用药过程观测裸鼠体质量、肿瘤体积和瘤重的变化。用免疫组织化学染色法来检测移植瘤组织中凋亡相关蛋白 NF-κB、Bcl-2和 Active caspase 3表达的变化。结果给药结束后，吉西他滨组的裸鼠平均体质量和对照组相比显著减小，而联合组中裸鼠平均体质量和对照组相比差异无统计学意义；联合组移植瘤平均体积和瘤重均明显比其他组小；E ＋G 组的 NF-κB、Bcl-2的表达与其余各组比较均明显降低，联合组中的 Active caspase 3的表达和其余各组相比明显增多。结论大黄素可显著提高吉西他滨对 Bxpc-3胰腺癌细胞裸鼠移植瘤的抑瘤作用，其作用机制可能为大黄素抑制凋亡抑制基因 NF-κB、Bcl-2的表达从而促进胰腺癌细胞的凋亡。
目的：探討大黃素增彊吉西他濱對胰腺癌細胞 Bxpc-3裸鼠移植瘤的促凋亡作用及其可能的作用機製。方法在培養人胰腺癌 Bxpc-3細胞的基礎上建立荷瘤模型，荷瘤裸鼠用隨機區組設計分配方法分為四組：0．9％氯化鈉註射溶液組（N 組）：腹腔註射0．9％氯化鈉註射溶液0．2 mL，大 黃 素 組（E 組，40 mg／kg），吉西他濱組（G 組，125 mg／kg），聯閤組（E ＋G 組，大黃素40 mg／kg 和吉西他濱80 mg／kg）。用藥均採用腹腔註射。用藥過程觀測裸鼠體質量、腫瘤體積和瘤重的變化。用免疫組織化學染色法來檢測移植瘤組織中凋亡相關蛋白 NF-κB、Bcl-2和 Active caspase 3錶達的變化。結果給藥結束後，吉西他濱組的裸鼠平均體質量和對照組相比顯著減小，而聯閤組中裸鼠平均體質量和對照組相比差異無統計學意義；聯閤組移植瘤平均體積和瘤重均明顯比其他組小；E ＋G 組的 NF-κB、Bcl-2的錶達與其餘各組比較均明顯降低，聯閤組中的 Active caspase 3的錶達和其餘各組相比明顯增多。結論大黃素可顯著提高吉西他濱對 Bxpc-3胰腺癌細胞裸鼠移植瘤的抑瘤作用，其作用機製可能為大黃素抑製凋亡抑製基因 NF-κB、Bcl-2的錶達從而促進胰腺癌細胞的凋亡。
목적：탐토대황소증강길서타빈대이선암세포 Bxpc-3라서이식류적촉조망작용급기가능적작용궤제。방법재배양인이선암 Bxpc-3세포적기출상건립하류모형，하류라서용수궤구조설계분배방법분위사조：0．9％록화납주사용액조（N 조）：복강주사0．9％록화납주사용액0．2 mL，대 황 소 조（E 조，40 mg／kg），길서타빈조（G 조，125 mg／kg），연합조（E ＋G 조，대황소40 mg／kg 화길서타빈80 mg／kg）。용약균채용복강주사。용약과정관측라서체질량、종류체적화류중적변화。용면역조직화학염색법래검측이식류조직중조망상관단백 NF-κB、Bcl-2화 Active caspase 3표체적변화。결과급약결속후，길서타빈조적라서평균체질량화대조조상비현저감소，이연합조중라서평균체질량화대조조상비차이무통계학의의；연합조이식류평균체적화류중균명현비기타조소；E ＋G 조적 NF-κB、Bcl-2적표체여기여각조비교균명현강저，연합조중적 Active caspase 3적표체화기여각조상비명현증다。결론대황소가현저제고길서타빈대 Bxpc-3이선암세포라서이식류적억류작용，기작용궤제가능위대황소억제조망억제기인 NF-κB、Bcl-2적표체종이촉진이선암세포적조망。
Objective To investigate the enhanced gemcitabine-induced apoptosis by emodin and the possi-ble therapeutic mechanism.Methods Human pancreatic carcinoma Bxpc-3 cells were cultured and the murine xeno-graft model bearing human pancreatic carcinoma was established.Then the mice were randomly divided into control group,emodin group,gemcitabine group and emodin combined with gemcitabine group.Body weight of mice、the tumor volume and tumor weight were measured during the study.And Immunohistochemistry(IHC)was used to detect the variance of the apoptotsis relative protein expression such as NF-κB、Bcl-2 and Active caspase 3.Results After the last administrations,the average weight of nude mice in gemcitabine group decreased significantly compared with the control group,and the nude mice weight in the combined therapy group had no significant decrease compared with the control group.And the mean tumor volume and tumor weight in combined administration group was significantly de-creased compared to the other groups.Immunohistochemistry(IHC)analysis showed the expression of NF-κB、bcl-2 proteins was significantly decreased in the combined administration group compared to the other gourps.Active caspase 3 was significantly increased compared to the other gourps.Conclusion Emodin can enhance the antitumor effect of gemcitabine in the growth of the xenografts of human pancreatic cancer Bxpc-3 cells in nude mice,whose mechanisms may be related to the downregulation of NF-κB and Bcl-2.
