CAJ | 학술논문

为提高贵州地方鸡种荔波瑶山鸡的产蛋性能，以催乳素基因 PRL 为候选基因之一，利用 PCR 方法直接检测催乳素基因 PRL 的5′-调控区24-bp插入／缺失（－377～－354bp）变异情况，并分析其与产蛋性能的关系。结果表明，荔波瑶山鸡该位点83．8％为缺失型，无插入纯合子；不同基因型（PRL＋／＋、＋／－和－／－型）对荔波瑶山鸡早期产蛋性能均无显著影响（P＞0．05）。
위제고귀주지방계충려파요산계적산단성능，이최유소기인 PRL 위후선기인지일，이용 PCR 방법직접검측최유소기인 PRL 적5′-조공구24-bp삽입／결실（－377～－354bp）변이정황，병분석기여산단성능적관계。결과표명，려파요산계해위점83．8％위결실형，무삽입순합자；불동기인형（PRL＋／＋、＋／－화－／－형）대려파요산계조기산단성능균무현저영향（P＞0．05）。
In an attempt to improve the egg-laying of the local Liboyaoshan chickens,PCR analysis was used todetect and identify the possible association of the 24-bp Indel (-3 7 7 to -3 54 bp)in PRL 5′-region with the egglayingof the chicken.The results showed that the site was a deletion type (8 3 %)and no homozytes of insertion wasfound.There was no significant differences on the egg-laying performance of the chickens with PRL+/+,+/- or-/- genotypes (P >0.05).