CAJ | 학술논문

采用高效液相色谱方法测定6种笃斯越桔制品中的有机酸含量，色谱条件为色谱柱：VenusilASBC18(4. 6mm×250mm, 5μm)；流动相：96. 9%超纯水-3%甲醇-0. 1%甲酸；流速：0. 5mL/min；柱温：室温；检测波长：210nm；进样量：20μm。该方法能够快速、准确地分离笃斯越桔相关制品中所含的7种有机酸，包括草酸、奎宁酸、苹果酸、莽草酸、乳酸、乙酸、柠檬酸。检测结果表明，笃斯越桔中的有机酸主要为奎宁酸，其次是柠檬酸和苹果酸，乳酸和乙酸的含量较少。
채용고효액상색보방법측정6충독사월길제품중적유궤산함량，색보조건위색보주：VenusilASBC18(4. 6mm×250mm, 5μm)；류동상：96. 9%초순수-3%갑순-0. 1%갑산；류속：0. 5mL/min；주온：실온；검측파장：210nm；진양량：20μm。해방법능구쾌속、준학지분리독사월길상관제품중소함적7충유궤산，포괄초산、규저산、평과산、망초산、유산、을산、저몽산。검측결과표명，독사월길중적유궤산주요위규저산，기차시저몽산화평과산，유산화을산적함량교소。
A H PLC m ethod w asdeveloped to determ ine organic acidsin 6 V accinium uliginosum products.The H PLC separation w asachieved on C 18 colum n (4.6 m m ×250 m m ,5 μm )atroom tem perature.The m obile phase w as96.9% ultrapure w ater-3% m ethanol-0.1% form ic acid ata flow rate of0.5 m l/m in.The detection w avelength w as setas 210 nm and sam ple size w as 20 μl.Seven organic acids could be successfully separated from V .uliginosum products using this m ethod,including oxalic acid,quinic acid,m alic acid,shikim ic acid,lactic acid,acetic acid and citric acid.The resultsindicated thatthe m ain organic acid existing in V .uliginosum productsw asquinic acid,follow ed by citric acid and m alic acid;the contentsof lactic acid and acetic acid w ere quite low .