中国酿造
中國釀造
중국양조
CHINA BREWING
2014年
11期
94-97
,共4页
黑曲霉%复合诱变%柠檬酸%条件优化
黑麯黴%複閤誘變%檸檬痠%條件優化
흑곡매%복합유변%저몽산%조건우화
A spergillusniger%com pound m utation%citric acid%conditionsoptim ization
为了选育高产柠檬酸菌种,以前期分离到的一株黑曲霉(A spergillusniger)Y Z-35为出发菌株,经紫外线(U V)与硫酸二乙酯(D ES)复合诱变后,进行了产柠檬酸发酵条件优化试验。结果表明,选育出黑曲霉U V -D E-3是一株优良高产菌株,该菌株的产酸率达到11.54%,比初始菌株(Y Z-35)产酸率提高了69.70%;以红薯为发酵原料,在初始糖含量12%、温度35℃、起始pH 值为6.5,转速160 r/m in、发酵时间72 h的优化条件下,摇瓶发酵平均产酸率达到14.24%。
為瞭選育高產檸檬痠菌種,以前期分離到的一株黑麯黴(A spergillusniger)Y Z-35為齣髮菌株,經紫外線(U V)與硫痠二乙酯(D ES)複閤誘變後,進行瞭產檸檬痠髮酵條件優化試驗。結果錶明,選育齣黑麯黴U V -D E-3是一株優良高產菌株,該菌株的產痠率達到11.54%,比初始菌株(Y Z-35)產痠率提高瞭69.70%;以紅藷為髮酵原料,在初始糖含量12%、溫度35℃、起始pH 值為6.5,轉速160 r/m in、髮酵時間72 h的優化條件下,搖瓶髮酵平均產痠率達到14.24%。
위료선육고산저몽산균충,이전기분리도적일주흑곡매(A spergillusniger)Y Z-35위출발균주,경자외선(U V)여류산이을지(D ES)복합유변후,진행료산저몽산발효조건우화시험。결과표명,선육출흑곡매U V -D E-3시일주우량고산균주,해균주적산산솔체도11.54%,비초시균주(Y Z-35)산산솔제고료69.70%;이홍서위발효원료,재초시당함량12%、온도35℃、기시pH 치위6.5,전속160 r/m in、발효시간72 h적우화조건하,요병발효평균산산솔체도14.24%。
In orderto breed high yield strains ofcitric acid,using the pre-isolated strain ofA spergillus nigerY Z-35 as starting strain afterultraviolet radiation and diethylsulfate com pound m utation,the experim entsofferm entation conditionsoptim ization forcitric acid production w ere carried out. The results show ed thatA .nigerU V -D E-3 w as strain w ith high citric acid production,the acid production rate reached 11.54% ,w hich w as 69.70%higherthan the initialstrain Y Z-35.U sing sw eetpotato as raw m aterial,underthe optim alcondition ofinitialsugarconcentration 12% ,tem perature 35 ℃,initialpH 6.5,rotated speed 160 r/m in,tim e 72 h,the average acid production rate by shake flask ferm entation w as14.24% .