CAJ | 학술논문

研究了蛋白酶对纤维素酶、淀粉酶活力的影响，结果表明淀粉酶对蛋白酶比较敏感，淀粉酶在质量浓度为150 U /m L的蛋白酶条件下37℃处理30 m in，相对酶活力为75.79%。研究了纤维素酶和淀粉酶的热稳定性，结果表明与纤维素酶相比，淀粉酶对高温更敏感，淀粉酶在100℃的沸水中处理1 m in，相对酶活力为1.89%。研究了金属离子对纤维素酶和淀粉酶的影响，结果表明，对于纤维素酶激活作用大小顺序依次为C u2+＞Zn2+＞C o2+＞M n2+＞Fe2+，抑制作用大小顺序依次为M n2+＞Fe2+＞C o2+＞Zn2+＞C u2+；对于淀粉酶激活作用不明显,抑制作用大小顺序依次为Fe2+＞C u2+＞M n2+＞Zn2+＞C o2+。以上研究的目的是为酶在饲料中的应用提供参考。
연구료단백매대섬유소매、정분매활력적영향，결과표명정분매대단백매비교민감，정분매재질량농도위150 U /m L적단백매조건하37℃처리30 m in，상대매활력위75.79%。연구료섬유소매화정분매적열은정성，결과표명여섬유소매상비，정분매대고온경민감，정분매재100℃적비수중처리1 m in，상대매활력위1.89%。연구료금속리자대섬유소매화정분매적영향，결과표명，대우섬유소매격활작용대소순서의차위C u2+＞Zn2+＞C o2+＞M n2+＞Fe2+，억제작용대소순서의차위M n2+＞Fe2+＞C o2+＞Zn2+＞C u2+；대우정분매격활작용불명현,억제작용대소순서의차위Fe2+＞C u2+＞M n2+＞Zn2+＞C o2+。이상연구적목적시위매재사료중적응용제공삼고。
The effectofprotease on the activity ofcellulase and am ylase w as studied,and resultshow ed thatam ylase w as m ore sensitive to protease than cellulase.The relative enzym atic activity ofam ylase w as75.79% w hen treated in 150 u/m lprotease at37 ℃ for30 m in.The therm ostabilitiesof cellulase and am ylase w ere tested and the resultsshow ed thatam ylase w asm ore sensitive to high tem perature than cellulase.The relative enzym e ac-tivity ofam ylase w as1.89% w hen treated in 100 ℃ w aterfor1 m in.Effectsofdifferentm etalionson the activity ofcellulase and am ylase w ere test-ed and the results show ed thatactivation effecton cellulase ranked in order w as C u2+,Zn2+,C o2+,M n2+ and Fe2+,the inhibition effecton cellulase ranked in orderw asM n2+,Fe2+,C o2+,Zn2+ and C u2+.A ctivation effecton am ylase w asnotdistinctand inhibition effecton am ylase ranked in orderw as Fe2+,C u2+,M n2+,Zn2+ and C o2+.The research purpose w asto provide a convenientreference forfurtherresearch aboutenzym e applicationsto feed.