中国酿造
中國釀造
중국양조
CHINA BREWING
2014年
11期
51-54
,共4页
吕和鑫%贾士儒%崔相敢%夏锋
呂和鑫%賈士儒%崔相敢%夏鋒
려화흠%가사유%최상감%하봉
杜氏盐藻%β-胡萝卜素%金属离子%消耗速率%基质消耗动力学
杜氏鹽藻%β-鬍蘿蔔素%金屬離子%消耗速率%基質消耗動力學
두씨염조%β-호라복소%금속리자%소모속솔%기질소모동역학
D unaliella salina%β-carotene%m etalion%consum ption rate%consum ption kinetics
通过原子吸收光谱测定了杜氏盐藻对钙、镁、钾、铁四种离子的消耗速率,构建金属离子含量限制型培养基,研究了补加金属离子对杜氏盐藻生长光合速率、色素含量的影响。结果表明,对数期杜氏盐藻细胞对铁离子与钙离子的消耗呈线性降低,每增殖106个细胞分别消耗0.042μg的铁离子与0.708μg的钙离子,而在稳定期后,钙、铁离子浓度继续下降。生长时期中钾离子与镁离子的含量变化不显著。铁、钙离子补料能恢复杜氏盐藻的生长,但光合活性与色素含量变化较补料前的对数期光合速率与色素含量要低,但总类胡萝卜素含量要高于补料前对数期的。培养到稳定期的培养基经过回收补加有限的铁、钙离子后,细胞生长延滞期消失,但稳定期细胞浓度降低。回流培养基对数期细胞的光合速率与叶绿素含量降低,但是总类胡萝卜素含量升高。
通過原子吸收光譜測定瞭杜氏鹽藻對鈣、鎂、鉀、鐵四種離子的消耗速率,構建金屬離子含量限製型培養基,研究瞭補加金屬離子對杜氏鹽藻生長光閤速率、色素含量的影響。結果錶明,對數期杜氏鹽藻細胞對鐵離子與鈣離子的消耗呈線性降低,每增殖106箇細胞分彆消耗0.042μg的鐵離子與0.708μg的鈣離子,而在穩定期後,鈣、鐵離子濃度繼續下降。生長時期中鉀離子與鎂離子的含量變化不顯著。鐵、鈣離子補料能恢複杜氏鹽藻的生長,但光閤活性與色素含量變化較補料前的對數期光閤速率與色素含量要低,但總類鬍蘿蔔素含量要高于補料前對數期的。培養到穩定期的培養基經過迴收補加有限的鐵、鈣離子後,細胞生長延滯期消失,但穩定期細胞濃度降低。迴流培養基對數期細胞的光閤速率與葉綠素含量降低,但是總類鬍蘿蔔素含量升高。
통과원자흡수광보측정료두씨염조대개、미、갑、철사충리자적소모속솔,구건금속리자함량한제형배양기,연구료보가금속리자대두씨염조생장광합속솔、색소함량적영향。결과표명,대수기두씨염조세포대철리자여개리자적소모정선성강저,매증식106개세포분별소모0.042μg적철리자여0.708μg적개리자,이재은정기후,개、철리자농도계속하강。생장시기중갑리자여미리자적함량변화불현저。철、개리자보료능회복두씨염조적생장,단광합활성여색소함량변화교보료전적대수기광합속솔여색소함량요저,단총류호라복소함량요고우보료전대수기적。배양도은정기적배양기경과회수보가유한적철、개리자후,세포생장연체기소실,단은정기세포농도강저。회류배양기대수기세포적광합속솔여협록소함량강저,단시총류호라복소함량승고。
The consum ption rate of D unaliella salina on iron,potassium ,calcium and m agnesium w ere determ ined by atom ic absorption spectrum . The m etalion contentlim ittype m edium w asestablished and the effectofadding m etalion on D .salina grow th,photosynthetic rate and pigm entcon-tentw ere studied.The results show ed thatthe consum ption of iron and calcium decreased linearly during the log grow th phase.The consum ption rates ofiron and calcium in the m edium w ere 0.042 μg and 0.708 μg for 106 cellproliferation,and after stable phase,calcium ,iron concentration continued to fall.H ow ever,the difference ofpotassium and m agnesium concentration in the m edium w as notsignificantin the grow th period.The feeding ofiron and calcium ionscould prom ote the grow th ofD .salina cellsto revive butw ith a low erphotosynthetic ratesand chlorophyllcontents, the totalcarotenoid contentw as higherthan logarithm ic phase before feeding.A fterrecycling adding lim ited iron and calcium to the m edium in the stable phase,the celllag phase disappeared butthe cellconcentration decreased in the stable phase.The photosynthetic rate and chlorophyllcontent ofcellin log phase in backflow m edium decreased,butthe totalcarotenoid contentincreased inversely.