CAJ | 학술논문

分离、鉴定赤水丹霞地区土壤来源的抗临床耐药菌活性的链霉菌。采集赤水丹霞山区不同海拔的土样并利用多种培养基分离、纯化菌株。热酚法提取菌株基因组DNA，PCR扩增16SrRNA基因并测序后在线比对以确定其系统分类学地位。利用多种测试菌测试抗菌活性，通过HPLC和LC/MS分析菌株发酵抽提物并与数据库比较。分离得到菌株600株，其中59株链霉菌中有41株具有1种以上的抗测试菌活性。Streptomycetessp. CSDX001具有抗耐药耐甲氧西林金黄色葡萄球菌、抗耐万古霉素金黄色葡萄球菌、抗多药耐药鲍曼不动杆菌、抗枯草芽孢杆菌、抗藤黄微球菌、抗白色念球菌的活性，具有抗多种微生物的活性和产生多种新结构次级代谢产物潜力，可能是抗微生物新天然活性产物的重要来源。
분리、감정적수단하지구토양래원적항림상내약균활성적련매균。채집적수단하산구불동해발적토양병이용다충배양기분리、순화균주。열분법제취균주기인조DNA，PCR확증16SrRNA기인병측서후재선비대이학정기계통분류학지위。이용다충측시균측시항균활성，통과HPLC화LC/MS분석균주발효추제물병여수거고비교。분리득도균주600주，기중59주련매균중유41주구유1충이상적항측시균활성。Streptomycetessp. CSDX001구유항내약내갑양서림금황색포도구균、항내만고매소금황색포도구균、항다약내약포만불동간균、항고초아포간균、항등황미구균、항백색념구균적활성，구유항다충미생물적활성화산생다충신결구차급대사산물잠력，가능시항미생물신천연활성산물적중요래원。
In orderto isolate and identify the Streptom ycetesspp.w ith antim icrobialactivity from C hishuiD anxia region,the soilsam plesfrom differ-entaltitudes w as collected,and then isolated and purified by a variety ofm edium .The strain gene D N A w as extracted by hotphenolm ethod,16S rR N A PC R products w as sequenced and blasted online for determ ine the phylogeny ofisolated strains.A ntim icrobialactivities w ere detected w ith m ultiple test bacteria,and the ferm entation extracts w ere analyzed by H PLC and LC /M S,and the results w ere com pared w ith database.R esults show ed that600 strains w ere isolated and 41 outof59 Streptom ycetes strains had m ore than one antim icrobialactivity.Streptom yces sp.C SD X 001 show ed resistance activity to m ethicillin-resistantStaphylococcus aureus,vancom ycin-resistantStaphylococcus aureus,m ultidrug resistantA cineto-bacter baum annii,B acillus subtilis,M icrococcus luteus,C andida albicans;itshow ed antim icrobialactivities and secondary m etabolites-producing potential,w hich m ightbe a potentialbacterialresource foractive naturalproductsresearch and developm ent.