CAJ | 학술논문

以传统发酵辣椒为分离源，从中分离出10株可降解亚硝酸盐的乳酸菌，经过复筛得到一株产亚硝酸盐还原酶能力最强的短乳杆菌（Lactobacillusbrevis）L5，并通过单因素及正交试验对其降解特性进行了研究。结果表明：L5对亚硝酸盐有明显的降解作用，在正常培养条件下，发酵36 h，L5对亚硝酸盐的降解率为56.25%；在维持pH 6.0以上，消除酸对亚硝酸盐降解作用的条件下，发酵36 h， L5对亚硝酸盐的降解率为66.42%，主要为产酶降解。L5降解亚硝酸盐的最佳条件为温度34℃，时间30 h，底物质量浓度120 m g/L，食盐含量6%，此时，L5对亚硝酸盐的降解率可达82.84%。
이전통발효랄초위분리원，종중분리출10주가강해아초산염적유산균，경과복사득도일주산아초산염환원매능력최강적단유간균（Lactobacillusbrevis）L5，병통과단인소급정교시험대기강해특성진행료연구。결과표명：L5대아초산염유명현적강해작용，재정상배양조건하，발효36 h，L5대아초산염적강해솔위56.25%；재유지pH 6.0이상，소제산대아초산염강해작용적조건하，발효36 h， L5대아초산염적강해솔위66.42%，주요위산매강해。L5강해아초산염적최가조건위온도34℃，시간30 h，저물질량농도120 m g/L，식염함량6%，차시，L5대아초산염적강해솔가체82.84%。
Ten lactic acid bacteria strains thatcan degrade nitrite w ere isolated from traditionalferm ented chili.O ne strain w hich had betterability of producing nitrite reductase w ere rescreened,nam ely Lactobacillus brevis L5,then single factor and orthogonaltests w ere adopted to study on its degradation characteristics.R esults show ed thatthe L5 had significantability for degrading nitrite,and under norm alculture condition,the nitrite degradation rate w as 56.25% after36 h ferm entation.In controlling pH above 6.0,elim inating the acid degradation,the nitrite degradation rate w as 66.42% ,m ainly asenzym atic degradation.The optim um conditionsfornitrite degradation ofthe L5 w astem perature 34 ℃,tim e 30 h,substrate con-centration 120 m g/L and saltconcentration 6% .In thiscondition,the nitrite degradation rate ofthe L5 w as82.84% .