CAJ | 학술논문

利用抑制消减杂交技术筛选 AM 真菌在侵染紫穗槐的过程中紫穗槐差异表达的基因，共获得47个unigenes，其中35个显著匹配。根据 GO分类信息、Blast注释信息和相关文献信息，显著匹配的35个 unigenes基因按功能分为防御胁迫(24%)、物质代谢(17%)、能量代谢(14%)、蛋白合成(11%)、信号转导(11%)、细胞结构构建(11%)、转录(6%)、蛋白折叠和降解(6%)8类。其中，胁迫防御相关基因和代谢相关基因所占比例最大，表明二者在 AM 真菌和紫穗槐共生互作过程中起重要作用。这些基因广泛参与生物体生命活动的各个过程，因此 AM真菌与紫穗槐共生的过程是一个系统涉及各种生物过程的活动。
이용억제소감잡교기술사선 AM 진균재침염자수괴적과정중자수괴차이표체적기인，공획득47개unigenes，기중35개현저필배。근거 GO분류신식、Blast주석신식화상관문헌신식，현저필배적35개 unigenes기인안공능분위방어협박(24%)、물질대사(17%)、능량대사(14%)、단백합성(11%)、신호전도(11%)、세포결구구건(11%)、전록(6%)、단백절첩화강해(6%)8류。기중，협박방어상관기인화대사상관기인소점비례최대，표명이자재 AM 진균화자수괴공생호작과정중기중요작용。저사기인엄범삼여생물체생명활동적각개과정，인차 AM진균여자수괴공생적과정시일개계통섭급각충생물과정적활동。
In this study, the suppression subtractive hybridization ( SSH ) method was employed to screen the differential expressed genes in Amorpha fruticosa roots induced by AMF during the colonization process. The results showed that 47 unigenes were obtained,and 35 of them could be aligned with known function genes in GenBank through Blast. According to GO classified information,Blast annotation and related literatures,the 35 unigenes were assigned into eight categories including stress and defense (24%) ,substance metabolism (17%) ,energy metabolism (14%) , protein synthesis ( 11%) ,signaling ( 11%) ,cellular structre/ cytoskeleton ( 11%) ,transcription related ( 6%) , protein folding and degradation related (6%) . Among them,stress and defense genes,and metabolism genes occupied the largest fraction,implying stress and defense related genes and substance metabolism related genes should play an important role in the symbiosis process between A. fruticosa and AMF. These genes are widely involved in the biological process of life activity,thus,the process of AMF and A. fruticosa symbiosis is a system involving a variety of biological activities.