CAJ | 학술논문

目的：探讨肿瘤坏死因子（TNF）‐α对甲醛诱导的炎症疼痛大鼠的疼痛症状及 TNF‐α的作用及机制。方法将45只雄性SD大鼠按照随机数字表法随机分为假手术组、模型组、依那西普组，每组15只。模型组和依那西普组大鼠左后足底掌部皮下注射2．5％甲醛溶液10μL进行造模，假手术组大鼠左后足底掌部皮下注射0．9％氯化钠注射液10μL进行对照。造模后依那西普组大鼠腹腔注射依那西普0．5 mg／kg进行干预，假手术组、模型组大鼠腹腔注射0．9％氯化钠注射液0．5 mg／kg进行对照。术后正常饲养大鼠。于术后1、7、14 d检测大鼠机械缩足反射阈值，14 d处死大鼠，用免疫组织化学法测定脊髓 TNF‐α的含量。结果假手术组TNF‐α的含量均低于模型组、依那西普组；模型组TNF‐α的含量术后增加明显，与假手术组比较，差异有统计学意义（ P＜0．05）；依那西普组TNF‐α的含量增加幅度小，与模型组比较，差异有统计学意义（ P＜0．05）。大鼠的机械缩足反射阈值，各组之内不同时间点之间总体均数差异无统计学意义（ P ＞0．05）；各组之间总体均数差异有统计学意义（P ＜0．05）；各时间点和组别之间的交互作用无统计学意义（P ＞0．05）。结论依那西普可以缓解炎性疼痛大鼠的疼痛症状，其机制与抑制TNF‐α的表达有关。
목적：탐토종류배사인자（TNF）‐α대갑철유도적염증동통대서적동통증상급 TNF‐α적작용급궤제。방법장45지웅성SD대서안조수궤수자표법수궤분위가수술조、모형조、의나서보조，매조15지。모형조화의나서보조대서좌후족저장부피하주사2．5％갑철용액10μL진행조모，가수술조대서좌후족저장부피하주사0．9％록화납주사액10μL진행대조。조모후의나서보조대서복강주사의나서보0．5 mg／kg진행간예，가수술조、모형조대서복강주사0．9％록화납주사액0．5 mg／kg진행대조。술후정상사양대서。우술후1、7、14 d검측대서궤계축족반사역치，14 d처사대서，용면역조직화학법측정척수 TNF‐α적함량。결과가수술조TNF‐α적함량균저우모형조、의나서보조；모형조TNF‐α적함량술후증가명현，여가수술조비교，차이유통계학의의（ P＜0．05）；의나서보조TNF‐α적함량증가폭도소，여모형조비교，차이유통계학의의（ P＜0．05）。대서적궤계축족반사역치，각조지내불동시간점지간총체균수차이무통계학의의（ P ＞0．05）；각조지간총체균수차이유통계학의의（P ＜0．05）；각시간점화조별지간적교호작용무통계학의의（P ＞0．05）。결론의나서보가이완해염성동통대서적동통증상，기궤제여억제TNF‐α적표체유관。
Objective To investigate the effects and mechanism of etanercept on pain symptoms and expres‐sions of tumor necrosis factor(TNF)‐αin rats model with inflammatory pain .Methods We equally randomized 45 male SD rats to groups A(sham operation) ,B(model) and C(Etanercept) .Group B and C received left foot injec‐tion 2.5% formaldehyde 10 μL to establish complete inflammatory pain rats model .In group A normal saline was given intraperitoneal injection instead of formaldehyde .After modeling ,the rats in group C received intraperitoneal injection of etanercept at 0 .5 mg/kg ,while those in groups A and B were intraperitonealy injectied with the same dose of normal saline .After building the model in 1 ,7 ,14 days ,mechanical withdrawal threshold was implemen‐ted .After 14 days ,the expression of TNF‐α was examined by immunohistochemistry .Results The levels of TNF‐αin group A were less than that in group B and C .There were significant differences in the levels of TNF‐αbetween groups B and A ( P<0 .05) .Compared with group B ,the increase in the levels of TNF‐αin group C was less than that in group B( P <0 .05).The mechanical withdrawal threshold analysis within each group ,different time points between the overall mean difference was of no statistical significance ( P>0.05);between the groups , the overall average difference has statistical significance ( P <0.05);each time point and between groups interact with no statistical significance ( P >0 .05) .Conclusion Etanercept administration protects the rats inflammatory pain .The mechanism may be inhibition of the overexpression of TNF‐α.