CAJ | 학술논문

目的：比较声门上型喉鳞状细胞癌与癌旁正常组织中缺氧诱导因子-1α（HIF-1α）、基质金属蛋白酶-1（MMP-1）和血管内皮生长因子-C（VEGF-C）的表达，探讨其与临床病理参数、颈淋巴转移之间的关系以及三者之间的相关性。方法免疫组化 SP 法检测30例声门上型喉鳞状细胞癌及其癌旁正常组织中 HIF-1α、MMP-1和VEGF-C 的表达情况，并对喉癌组织按年龄、T 分期、病理分级、淋巴转移进行分组，探讨三者与各临床病理参数、颈淋巴转移之间的关系。结果喉鳞状细胞癌组织中 HIF-1α、MMP-1、VEGF-C 高表达明显高于癌旁正常组织，差异有统计学意义（P <0.05），在癌组织中，三者表达均与病理分级、TNM 分期有关（ P <0.05），与年龄无关（ P >0.05），三者在淋巴结转移组中的高表达率明显高于未转移组，HIF-1α和 VEGF-C、HIF-1α和 MMP-1、MMP-1和VEGF-C 均呈正相关。结论喉鳞癌中 HIF-1α、MMP-1、VEGF-C 的高表达与颈淋巴转移密切相关，三者可能成为预测喉鳞癌转移和预后的生物学指标。
목적：비교성문상형후린상세포암여암방정상조직중결양유도인자-1α（HIF-1α）、기질금속단백매-1（MMP-1）화혈관내피생장인자-C（VEGF-C）적표체，탐토기여림상병리삼수、경림파전이지간적관계이급삼자지간적상관성。방법면역조화 SP 법검측30례성문상형후린상세포암급기암방정상조직중 HIF-1α、MMP-1화VEGF-C 적표체정황，병대후암조직안년령、T 분기、병리분급、림파전이진행분조，탐토삼자여각림상병리삼수、경림파전이지간적관계。결과후린상세포암조직중 HIF-1α、MMP-1、VEGF-C 고표체명현고우암방정상조직，차이유통계학의의（P <0.05），재암조직중，삼자표체균여병리분급、TNM 분기유관（ P <0.05），여년령무관（ P >0.05），삼자재림파결전이조중적고표체솔명현고우미전이조，HIF-1α화 VEGF-C、HIF-1α화 MMP-1、MMP-1화VEGF-C 균정정상관。결론후린암중 HIF-1α、MMP-1、VEGF-C 적고표체여경림파전이밀절상관，삼자가능성위예측후린암전이화예후적생물학지표。
Objective To compare the expression of HIF-1α,the MMP-1 and VEGF-C in laryn-geal squamous cell carcinoma(LSCC)and normal tissues around the cancer,and investigate the relation between the three factors with clinicopathological parameters and lymphatic metastasis. Methods HIF-1α、MMP-1 and VEGF-C gene protein in 30 cases of laryngeal carcin carcinoma and normal tissues a-round the cancer were detected by SP immunochemical technique,HIF-1α、MMP-1 and VEGF-C protein in 30 cases of laryngeal carcin carcinoma and the tissues around the cancer were detected. The relation-ship between the three factors and clinicopathological parameters and lymphatic metastasis were investiga-ted. The laryngeal carcinoma were devided by age,T tage,phathologic stage and lymphatic metastasis. Results Expression of HIF-1α,MMP-1,VEGF-C was significantly higher in laryngeal carcinoma than that in normal tissue around the cancer. Expression of the three factors were correlated with T stage and the pathologic stage( P < 0. 05 ),not with age( P > 0. 05),The over expression of HIF-1α、MMP-1、VEGF-C was related to cervical lymph nodes metastasis in two experiments. Correlation was found within VEGF-C and HIF-1α,HIF-1α and MMP-1,VEGF-C and MMP-1( P < 0. 05 ). Conclusions The over expression of HIF-1α,MMP-1,VEGF-C in laryngeal carcinoma was related to cervical lymph nodes metastasis,which suggested that they would become a biologic indexes to prognosis of laryngeal carcinoma.