CAJ | 학술논문

建立固相萃取-高效液相色谱法测定水质中16种多环芳烃，研究不同色谱条件对16种多环芳烃分离效果的影响，得出最佳分离条件；并通过系统考察样品的流速、洗脱条件和有机改性剂加入量对测定的影响，得出最佳固相萃取条件。研究结果表明，利用ZORBAX ECLIPSE PAH色谱分离专用柱，以甲醇和水为流动相，采用梯度淋洗和变化的紫外、荧光程序可以很好分析16种多环芳烃，分离度均在2.0以上；采用固相萃取-高效液相色谱法测定水中16种多环芳烃，5种不同水质的加标回收率均可达60%-110%，且准确度和精密度较高，RSD均小于10%，标准曲线线性均能在0.999以上。同时，采用荧光为检测器，本方法中目标化合物的方法检出限为10-200 pg/L，可以很好满足目前监测要求。
건립고상췌취-고효액상색보법측정수질중16충다배방경，연구불동색보조건대16충다배방경분리효과적영향，득출최가분리조건；병통과계통고찰양품적류속、세탈조건화유궤개성제가입량대측정적영향，득출최가고상췌취조건。연구결과표명，이용ZORBAX ECLIPSE PAH색보분리전용주，이갑순화수위류동상，채용제도림세화변화적자외、형광정서가이흔호분석16충다배방경，분리도균재2.0이상；채용고상췌취-고효액상색보법측정수중16충다배방경，5충불동수질적가표회수솔균가체60%-110%，차준학도화정밀도교고，RSD균소우10%，표준곡선선성균능재0.999이상。동시，채용형광위검측기，본방법중목표화합물적방법검출한위10-200 pg/L，가이흔호만족목전감측요구。
A method of solid phase extraction and high performance liquid chromatography determination of 16 polycyclic aromatic hydrocarbons (PAHs) in water has been established. In order to acquire the best conditions, various parameters were studied, like as the deferent parameters of liquid chromatography, the sample flow rates, eluent volume and organic modifier concentration. All of the components were separated completely from each other on the special chromatographic column of ZORBAX ECLIPSE PAH with methanol-water gradient elution, and UV-detector and lfuorescence detector. In addition, the average recoveries of 16 PAHs in ifve kinds of water ranged from 60%-110%, their relative standard deviation was less than 10% and the correlation of linearity was greater than 0.999 all. The method developed is selective, sensitive and accurate, the detection limit for 15 PAHs were from 10-200 pg/L with lfuorescence detector, the method completely suitable for determination of PAHs analysis in water.