CAJ | 학술논문

目的：体外探索促红细胞生成素（EPO）拮抗环孢素 A（CsA）所致肾小管上皮损伤的作用机制。方法采用人近曲肾小管上皮细胞作为体外实验模型，实验分对照组、CsA对照组、EPO 15 U／mL与 CsA合用组、EPO 30 U／mL与CsA合用组，MTT法检测细胞增殖抑制率，采用金氏公式评价 EPO与 CsA合用拮抗作用效果，采用 ELISA方法检测细胞培养上清液中肾损伤分子-1（KIM-1）的表达量，采用流式细胞仪检测细胞生存情况。结果采用金氏公式评价结果显示，EPO在15 U／mL与30 U／mL浓度时可以拮抗 CsA对肾小管上皮的损伤作用；对比 CsA对照组，EPO在15 U／mL与30 U／mL浓度可降低细胞培养上清液中 KIM-1的表达量（P＜0．05），降低凋亡细胞比率（P＜0．05），降低坏死细胞比率（P＜0．05），增加活细胞比率（P＜0．05）。结论 EPO拮抗CsA所致肾小管上皮损伤，可能通过减少细胞凋亡而实现。
목적：체외탐색촉홍세포생성소（EPO）길항배포소 A（CsA）소치신소관상피손상적작용궤제。방법채용인근곡신소관상피세포작위체외실험모형，실험분대조조、CsA대조조、EPO 15 U／mL여 CsA합용조、EPO 30 U／mL여CsA합용조，MTT법검측세포증식억제솔，채용금씨공식평개 EPO여 CsA합용길항작용효과，채용 ELISA방법검측세포배양상청액중신손상분자-1（KIM-1）적표체량，채용류식세포의검측세포생존정황。결과채용금씨공식평개결과현시，EPO재15 U／mL여30 U／mL농도시가이길항 CsA대신소관상피적손상작용；대비 CsA대조조，EPO재15 U／mL여30 U／mL농도가강저세포배양상청액중 KIM-1적표체량（P＜0．05），강저조망세포비솔（P＜0．05），강저배사세포비솔（P＜0．05），증가활세포비솔（P＜0．05）。결론 EPO길항CsA소치신소관상피손상，가능통과감소세포조망이실현。
Objective To investigate the effect of Erythropoietin (EPO)on the human renal tubular cells injured by cyclosporin A (CsA)and its mechanism.Methods human renal proximal tubular epithelial cells was used as in vitro model,experiments were divided into control group,CsA group,EPO group,15 U/mL combined with CsA EPO 30 U/mL combined with CsA group,the cell proliferation was detected by MTT inhibition rate,using EPO and CsA's formula evaluation combined with antagonistic effect,detection of cell culture supernatant of kidney inj ury molecule in-1 by u-sing the method of ELISA (KIM-1)expression,the cell viability was detected by flow cytometry.Results the Jin's for-mula evaluation results show that,EPO can antagonize the effect of CsA on the inj ury of renal tubular epithelium in 1 5 U/mL and 30 U/mL concentration;compared with CsA group,EPO could decrease the expression of KIM-1 in the su-pernatants of cells cultured in 15 U/mL and 30 U/mL concentration (P<0.05),decrease apoptotic cell ratio (P<0.05),reduce the ratio of necrotic cells (P<0.05),increasing the ratio of living cells (P<0.05).Conclusion EPO an-tagonized the CsA induced renal tubular epithelial inj ury,may be achieved by reducing cell apoptosis.