CAJ | 학술논문

目的：探讨丹参磷脂浸膏（DSLZ）对实验小鼠肝损伤的保护作用。方法 ICR小鼠60只，随机分为空白对照组、模型组及丹参磷脂浸膏高、中、低剂量组和阳性对照丹参酮 IIA（SalviolⅡA IIA）组，采用四氯化碳（CCl4）法建立急性肝损伤实验动物模型，观察丹参磷脂浸膏对实验小鼠血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、丙二醛（MDA）、还原型谷胱甘肽（GSH）以及肝组织病理改变的影响。结果与空白对照组比较，模型组小鼠血清ALT、AST及MDA均显著增高（P＜0．01），GSH明显降低（P＜0．01）。丹参磷脂浸膏高、中剂量组ALT、AST及 MDA明显降低（P＜0．05或P＜0．01），GSH明显升高（P＜0．05或P＜0．01）。HE染色显示丹参磷脂浸膏组小鼠肝组织病理损伤减轻。结论丹参磷脂浸膏对小鼠急性四氯化碳性肝损伤具有明显的抑制作用，其机制可能与抗氧化有关。
목적：탐토단삼린지침고（DSLZ）대실험소서간손상적보호작용。방법 ICR소서60지，수궤분위공백대조조、모형조급단삼린지침고고、중、저제량조화양성대조단삼동 IIA（SalviolⅡA IIA）조，채용사록화탄（CCl4）법건립급성간손상실험동물모형，관찰단삼린지침고대실험소서혈청병안산안기전이매（ALT）、천문동안산안기전이매（AST）、병이철（MDA）、환원형곡광감태（GSH）이급간조직병리개변적영향。결과여공백대조조비교，모형조소서혈청ALT、AST급MDA균현저증고（P＜0．01），GSH명현강저（P＜0．01）。단삼린지침고고、중제량조ALT、AST급 MDA명현강저（P＜0．05혹P＜0．01），GSH명현승고（P＜0．05혹P＜0．01）。HE염색현시단삼린지침고조소서간조직병리손상감경。결론단삼린지침고대소서급성사록화탄성간손상구유명현적억제작용，기궤제가능여항양화유관。
Objective To explore the protective effect of DSLZ on acute chemical liver injury induced by 1% carbon Tetrachloride (CCl4 )in mice and to reveal its mechanism.Methods 60 mice were randomly divided into normal control group,model group,SalviolⅡA group,high dose DSLZ group (2.6 mg/kg),middle dose group (1.56 mg/kg)and low dose group (0.52 mg/kg).All the mice were intragastrically administered the corresponding agents respectively for 30 days.After the last administration 1% CCl4 2.0 ml/kg was given to mice in all groups except control group in a single administration of vegetable oil intragastrically to establish the acute liver inj ury mouse model.1 6 h later,the mice were sacrificed,alanine aminotransferase (ALT),aspartate aminotrsferase (AST),malondialdehyde (MDA),reduced gluta-thione hormone (GSH)level in serum were detected.HE staining was performed for observing the pathological changes of the liver.Results Compared with the control group,the level of ALT,AST,MDA in the serum of the model group mice were increased significantly,and the GSH level was decreased significantly (P<0.01 ).Compared with model group,the serum ALT,AST,MDA level in hign dose of DSLZ and middle dose of DSLZ groups were decreased signifi-cantly (P<0.05 or P<0.01),while the GSH level in serum were increased significantly (P<0.05 or P<0.01).Mo-reover the HE staining results showed that in model group the swelling,kytoplasm coacervation,hydropic degeneration and necrosis of liver cells were seen.The morphological damage of liver cells were improved significantly in DSLZ groups.Conclusion DSLZ has a significant protective effect on CCl4 ! induced acute liver inj ury,which might be relat-ed to the anti-oxidation.