CAJ | 학술논문

运用石蜡切片技术、光学显微镜和扫描电子显微镜技术对鲁梅克斯长叶-20花芽形态分化、大小孢子发生与雌雄配子体发育以及胚后发育等生殖过程进行研究。结果表明，鲁梅克斯长叶-20花芽分化分为花序原基分化期、小花原基分化初期、萼片原基分化期、花瓣原基分化期、雄蕊原基形成期和雌蕊原基形成期；小孢子母细胞经减数分裂形成四分体，属四面体型，成熟花粉粒以2-细胞型为主，兼有3-细胞型，具有3条或4条萌发沟；雌蕊发育过程中双珠被的倒生胚珠、厚珠心发育形成核型胚乳，大孢子母细胞减数分裂后形成的四分体呈线形排列，合点端的一个功能大孢子发育成单核胚囊，经三次连续的有丝分裂形成八核的蓼型胚囊；受精后初生胚乳核先于合子分裂，为胚的发育提供营养，胚经过原胚期、球形胚期、心形胚期直至发育成具有完整子叶的成熟胚。
운용석사절편기술、광학현미경화소묘전자현미경기술대로매극사장협-20화아형태분화、대소포자발생여자웅배자체발육이급배후발육등생식과정진행연구。결과표명，로매극사장협-20화아분화분위화서원기분화기、소화원기분화초기、악편원기분화기、화판원기분화기、웅예원기형성기화자예원기형성기；소포자모세포경감수분렬형성사분체，속사면체형，성숙화분립이2-세포형위주，겸유3-세포형，구유3조혹4조맹발구；자예발육과정중쌍주피적도생배주、후주심발육형성핵형배유，대포자모세포감수분렬후형성적사분체정선형배렬，합점단적일개공능대포자발육성단핵배낭，경삼차련속적유사분렬형성팔핵적료형배낭；수정후초생배유핵선우합자분렬，위배적발육제공영양，배경과원배기、구형배기、심형배기직지발육성구유완정자협적성숙배。
Using the paraffin section technique, light microscope and scanning electron microscope (sem) technique to study Rumex flower bud morphological differentiation, megasporogenesis and microsporogenesis, male and female gametophyte development, and post-embryonic development. The results showed that the whole flower bud differentiation process could be divided into inflorescence primordia differentiation stage, initial differentiation stage, initial sepal primordium differentiation stage, petal primordium differentiation stage, stamen primordium formation differentiation stage, and pistil primordium formation differentiation stage;the tetrad formed by meiosis of microspore mother cel belonged to tetrahedral shape type; with three or four germination grooves, most mature pol en grains were 2-cel type, few were 3-cel type;the tetrad from meiosis of megaspore mother cel was linear;the chalazal megaspore developed into mononuclear embryo sac which further developed into eight nuclear embryo sac of Polygonum type by three successive mitotic; after fertilization, the primary endosperm nucleus division preceded zygote division to provide nutrition for embryo development; after proembryo, globular embryo, and heart-shaped embryo stage, a mature embryo with complete cotyledons was formed.