CAJ | 학술논문

以新疆2013年前审定的51个新陆早常规棉花品种为材料,从5000对 SSR 引物中筛选出多态性高、稳定性好、且定位在棉花26条染色体上(每条染色体上选择2~3对)的75对核心引物,检测到多态性位点226个,每个标记检测到的基因型位点数在2~12之间,平均为3.01个；引物多态信息量(PIC)值介于0.0799~0.8752之间,平均值为0.6624。结果显示,在51份新陆早棉花常规品种中,可利用特征引物将21份品种一次性区分开。利用40对引物可以完全区分开新陆早51份常规品种,并构建供试品种的指纹图谱。同时利用 NTSYS-pcV2.10软件聚类分析表明,51个新陆早棉花品种遗传相似系数变化范围为0.4269~0.9873,平均值为0.7071,说明新陆早棉花品种之间遗传多样性较狭窄；遗传相似系数矩阵和聚类分析将51个新陆早品种分为4大类型,与原品种选育系谱高度吻合。
이신강2013년전심정적51개신륙조상규면화품충위재료,종5000대 SSR 인물중사선출다태성고、은정성호、차정위재면화26조염색체상(매조염색체상선택2~3대)적75대핵심인물,검측도다태성위점226개,매개표기검측도적기인형위점수재2~12지간,평균위3.01개；인물다태신식량(PIC)치개우0.0799~0.8752지간,평균치위0.6624。결과현시,재51빈신륙조면화상규품충중,가이용특정인물장21빈품충일차성구분개。이용40대인물가이완전구분개신륙조51빈상규품충,병구건공시품충적지문도보。동시이용 NTSYS-pcV2.10연건취류분석표명,51개신륙조면화품충유전상사계수변화범위위0.4269~0.9873,평균치위0.7071,설명신륙조면화품충지간유전다양성교협착；유전상사계수구진화취류분석장51개신륙조품충분위4대류형,여원품충선육계보고도문합。
Fifty-one Xinluzao conventional cotton varieties authorized before 2013 were detected with 75 pairs of primers with high polymorphism, good repeatability, and even distribution on 26 chromosomes (2–3 pairs from each chromosome) selected from 5000 pairs of SSR primers. A total of 226 polymorphic loci were obtained, and each marker detected 2–12 polymorphic loci with an average of 3.01; the polymorphism information content ranged from 0.0799 to 0.8752, with an average of 0.6624. The results showed that 21 varieties were differentiated by characteristic primers, and the rest 30 varieties could be identified by primer conbinations. In the end, 40 specific primers and combinated primers could completely differentiate the 51 Xinluzao cotton varieties from each other. DNA fingerprinting of the 51 Xinluzao conventional varieties were constructed with the 40 pairs of SSR markers. NTSYS-pcV2.10 software was used to analyze genetic diversity of the 51 conventional varieties, the results indicated that genetic similarity coefficient for the varieties ranged from 0.4269 to 0.9873, with an average of 0.7071, showing the narrow genetic diversity in Xinluzao cotton varieties. The 51 Xinluzao cotton varieties were divided into four types with the genetic simi-larity coefficient matrix and cluster analysis, which were strongly consistent with their pedigrees.