CAJ | 학술논문

建立了高效液相色谱法准确、快速、灵敏测定化妆品中11种紫外吸收剂的方法。采用C18色谱柱,以乙腈和0.1％甲酸作为流动相,311 nm作为检测波长,在11.5 min内完成11种紫外吸收剂的基线分离。在优化的实验条件下,目标化合物的保留时间和峰面积的相对标准偏差分别小于0.05％和1.20％；另外,所有目标化合物的检出限均低于2.24 mg/L,并在5~500 mg/L 内均具有良好的线性关系(R2>0.9990),样品加标回收率为77％~116％。上述结果表明,本方法具有简便、准确、灵敏的特点。对两种不同化妆品中2-羟基-4甲氧基二苯甲酮-5-磺酸、对-氨基苯甲酸等11种紫外吸收剂的测定结果表明,2-羟基-4-甲氧基二苯甲酮、丁基甲氧基二苯酰基甲烷、水杨酸-2-乙基己酯、3,3,5-三甲基环已烷水杨酸酯4种紫外吸收剂在具有防晒功能的化妆品中较为常见,其中3,3,5-三甲基环已烷水杨酸酯在所测化妆品中浓度最高。
건립료고효액상색보법준학、쾌속、령민측정화장품중11충자외흡수제적방법。채용C18색보주,이을정화0.1％갑산작위류동상,311 nm작위검측파장,재11.5 min내완성11충자외흡수제적기선분리。재우화적실험조건하,목표화합물적보류시간화봉면적적상대표준편차분별소우0.05％화1.20％；령외,소유목표화합물적검출한균저우2.24 mg/L,병재5~500 mg/L 내균구유량호적선성관계(R2>0.9990),양품가표회수솔위77％~116％。상술결과표명,본방법구유간편、준학、령민적특점。대량충불동화장품중2-간기-4갑양기이분갑동-5-광산、대-안기분갑산등11충자외흡수제적측정결과표명,2-간기-4-갑양기이분갑동、정기갑양기이분선기갑완、수양산-2-을기기지、3,3,5-삼갑기배이완수양산지4충자외흡수제재구유방쇄공능적화장품중교위상견,기중3,3,5-삼갑기배이완수양산지재소측화장품중농도최고。
An accurate, fast and sensitive method based on high performance liquid chromatography was established for the simultaneous determination of eleven ultra-violet absorbents in cosmetics. Eleven ultra-violet absorbents were baseline separated on an Acclaim C18 column within 11. 5 min using acetonitrile-0. 1%formic acid in water ( V/V) mobile phase, and detected at 361 nm with UV detection. Under the optimized conditions, the relative standard derivations ( RSDs) of the eleven ultra-violet absorbents were all less than 0. 1% for retention time, and less than 1. 2% for peak area; good linearity was obtained from 5 to and 500 mg/L with the correlation coefficients of above 0 . 9990 for these analytes; the recoveries spiked in a cosmetic sample were in the range of 77% -116%. Benzophenone-3, butylmethoxydibenzoylmethane, ethylhexylsalicylatec and homosalate were found in the detected cosmetic samples, and the concentration of homosalate was the highest. The results indicated that this method had potential for applications due to its convenience, accuracy and sensitivity. Oxybenzone, butylmethoxydibenzoylmethane, 2-ethylhexyl salicylatec and homosalate were found in the detected cosmetic samples, and the concentration of homosalate was the highest.