CAJ | 학술논문

本试验旨在研究中华鳖( Trionyx sinensis)幼鳖对蚕蛹、发酵豆粕、肉骨粉和玉米蛋白粉4种饲料原料中粗蛋白质和氨基酸的表观消化率，以寻求中华鳖新型蛋白质饲料原料。试验饲料由70%的基础饲料和30%的待测饲料原料组成，并以0.5%的三氧化二铬(Cr2O3)作为外源指示剂。选取平均体重为(120.0±1.5) g的中华鳖幼鳖150只，随机分成5组(基础饲料组饲喂基础饲料，其他4组分别饲喂1种试验饲料)，每组3个重复，每个重复10只，饲养1周后开始用虹吸法收集粪便，进行粗蛋白质和氨基酸表观消化率的测定。结果表明：1)中华鳖幼鳖对肉骨粉、发酵豆粕、蚕蛹中粗蛋白质的表观消化率分别为90.57%、86.34%、85.75%，显著高于玉米蛋白粉的75.98%( P<0.05)。2)动物性蛋白质源中，中华鳖幼鳖对蚕蛹中总氨基酸的表观消化率较高，为92.44%，对肉骨粉中总氨基酸的表观消化率较低，仅为82.48%；植物性蛋白质源中，中华鳖幼鳖对玉米蛋白粉中总氨基酸的表观消化率较高，为96.56%，对发酵豆粕中总氨基酸的表观消化率为87.90%。综合分析得出，蚕蛹可作为中华鳖幼鳖的优质动物性蛋白质源，发酵豆粕可作为中华鳖幼鳖较好的植物性蛋白质源。
본시험지재연구중화별( Trionyx sinensis)유별대잠용、발효두박、육골분화옥미단백분4충사료원료중조단백질화안기산적표관소화솔，이심구중화별신형단백질사료원료。시험사료유70%적기출사료화30%적대측사료원료조성，병이0.5%적삼양화이락(Cr2O3)작위외원지시제。선취평균체중위(120.0±1.5) g적중화별유별150지，수궤분성5조(기출사료조사위기출사료，기타4조분별사위1충시험사료)，매조3개중복，매개중복10지，사양1주후개시용홍흡법수집분편，진행조단백질화안기산표관소화솔적측정。결과표명：1)중화별유별대육골분、발효두박、잠용중조단백질적표관소화솔분별위90.57%、86.34%、85.75%，현저고우옥미단백분적75.98%( P<0.05)。2)동물성단백질원중，중화별유별대잠용중총안기산적표관소화솔교고，위92.44%，대육골분중총안기산적표관소화솔교저，부위82.48%；식물성단백질원중，중화별유별대옥미단백분중총안기산적표관소화솔교고，위96.56%，대발효두박중총안기산적표관소화솔위87.90%。종합분석득출，잠용가작위중화별유별적우질동물성단백질원，발효두박가작위중화별유별교호적식물성단백질원。
The apparent digestibility of crude protein and amino acids of 4 feed ingredients—silkworm chrysa-lis, fermented soybean meal, meat-bone meal and corn gluten meal for juvenile soft-shelled turtle ( Trionyx sinensis) were determined in this experiment, in order to explore the new type protein feed ingredients for soft-shelled turtle. Four experimental diets were formulated. The experimental diets were consisted of 70% basal diet and 30% test feed ingredient, and contained 0.5% chromic oxide as an exogenous indicator. One hundred and fifty juvenile soft-shelled turtle with the average body weight of (120.0±1.5) g were randomly divided into 5 groups with 3 replicates per group and 10 soft-shelled turtle per replicate. The soft-shelled turtle in basal diet group were fed the basal diet, and those in the other 4 groups were fed one of experimental diets, respectively. After 1 weeks feeding, the fecal samples were collected by siphon method, and then the apparent digestibility of crude protein and amino acids was determined. The results showed as follows:1) the apparent digestibility of crude protein in corn gluten meal, fermented soybean meal and silkworm chrysalis for soft-shelled turtle was 90.57%, 86.34% and 85.75%, respectively, higher than that in meat-bone meal (75.98%) (P<0.05). 2) For animal protein sources, the apparent digestibility of total amino acids in silkworm chrysalis was higher, which was 92. 44%, while that in meat-bone meal was lower, which only was 75. 39%. For plant protein sources, the apparent digestibility of total amino acids in corn gluten meal was higher, which was 96.56%, and that in fermented soybean meal was 87.90%. The results indicate that silkworm chrysalis can be used as a source of high quality animal protein source, and fermented soybean meal can be used as a better plant protein source for soft-shelled turtle.