CAJ | 학술논문

本试验旨在研究饲粮添加黄芪多糖对大肠杆菌( O55∶B5)脂多糖诱导的肉鸡免疫应激状态下蛋白质重分配的调节作用。试验采用2×2双因素设计将1日龄爱拔益加肉鸡144只随机分为4组，每组6个重复，每个重复6只鸡。2个试验因素分别为：免疫应激(注射生理盐水或脂多糖)和饲粮黄芪多糖(0或3 g/kg)。21~35日龄进行蛋白质沉积试验。结果显示，免疫应激显著降低了肉鸡平均日采食量、平均日增重、左侧胸肌的蛋白质沉积量及沉积率( P<0.05)，同时显著提高了脾脏指数、脾脏蛋白质沉积量和脾脏、法氏囊的蛋白质沉积率( P<0.05)。而饲粮添加黄芪多糖可使肉鸡在免疫应激时，平均日采食量和平均日增重分别提高16.30%和18.34%，左侧胸肌的蛋白质沉积量和沉积率分别提高30.36%和12.34%，且显著抑制了脾脏蛋白质沉积量和沉积率的上升( P<0.05)。综上所述，在肉鸡免疫应激状态下，饲粮中添加3 g/kg黄芪多糖可抑制蛋白质从骨骼肌向免疫系统重分配，缓解肉鸡生长抑制。
본시험지재연구사량첨가황기다당대대장간균( O55∶B5)지다당유도적육계면역응격상태하단백질중분배적조절작용。시험채용2×2쌍인소설계장1일령애발익가육계144지수궤분위4조，매조6개중복，매개중복6지계。2개시험인소분별위：면역응격(주사생리염수혹지다당)화사량황기다당(0혹3 g/kg)。21~35일령진행단백질침적시험。결과현시，면역응격현저강저료육계평균일채식량、평균일증중、좌측흉기적단백질침적량급침적솔( P<0.05)，동시현저제고료비장지수、비장단백질침적량화비장、법씨낭적단백질침적솔( P<0.05)。이사량첨가황기다당가사육계재면역응격시，평균일채식량화평균일증중분별제고16.30%화18.34%，좌측흉기적단백질침적량화침적솔분별제고30.36%화12.34%，차현저억제료비장단백질침적량화침적솔적상승( P<0.05)。종상소술，재육계면역응격상태하，사량중첨가3 g/kg황기다당가억제단백질종골격기향면역계통중분배，완해육계생장억제。
This study was conducted to investigate the effects of Astragalus polysaccharide ( APS) on protein reallocation in broilers after challenge with E. coli ( O55∶B5) lipopolysaccharide ( LPS) . A total of 144 one-day-old Arbor Acres broilers were randomly divided into four groups ( six replicates per group and six birds per replicate) in terms of a 2×2 factorial design. The main factors were immune stress ( LPS or saline injection) and dietary APS ( 0 or 3 g/kg) . A trial of protein deposition was performed from 21 to 35 days of age. The re-sults showed that immune stress significantly reduced average daily feed intake ( ADFI ) , average daily gain ( ADG) , protein deposition ( PD) and protein deposition rate ( PDR) of the left pectoralis ( P<0.05) . Mean-while, the spleen index, spleen PD, spleen and bursa of fabricius PDRs were significantly increased by im-mune stress ( P<0. 05 ) . Under immune stress, however, ADFI and ADG were increased by 16. 30% and 18.34% respectively via dietary APS supplementation. The left pectoralis PD and PDR were increased by 30.36% and 12.34% respectively as well. The elevated spleen PD and PDR caused by immune stress were sig-nificantly decreased by dietary APS supplementation (P<0.05). In conclusion, dietary supplementation with 3 g/kg APS can inhibit protein redistribution from skeletal muscle to the immune system, and attenuate growth inhibition caused by immune stress.