CAJ | 학술논문

本试验旨在研究β-葡聚糖酶、木聚糖酶、纤维素酶和植酸酶用于肉鸡小麦型饲粮的最佳复合酶谱。采用单因素完全随机试验设计,将不同水平的β-葡聚糖酶(30、60、90、120、150和180 U/g)、木聚糖酶(200、400、600、800、1000和1200 U/g)、纤维素酶(200、400、600、800、1000和1200 U/g)和植酸酶(500、1000、1500、2000、2500和3000 U/kg)分别添加于肉鸡小麦型饲粮中,采用模拟胃肠液体外消化试验,研究单酶的最佳添加水平；据此,采用4因子3水平L9(34)正交设计和体外法研究4种单酶复配效应,每种酶各设计3个添加水平：β-葡聚糖酶为100、150和200 U/g,木聚糖酶为900、950和1000 U/g,纤维素酶为900、950和1000 U/g,植酸酶为1500、2000和2500 U/kg,以还原糖生成量、植酸磷降解率、饲料残渣总能为判定指标,确定4种单酶的最佳复合酶谱。结果表明：1~3周龄肉鸡小麦型饲粮中,当β-葡聚糖酶、木聚糖酶、纤维素酶添加水平分别为150、960、950 U/g 时,分别获得最大还原糖生成量0.918、1.161、0.927 mg/g,当植酸酶添加水平为2010 U/kg时,获得最大植酸磷降解率92.35%；4~6周龄肉鸡小麦型饲粮中,当β-葡聚糖酶、木聚糖酶、纤维素酶添加水平分别为150、950、960 U/g时,分别获得最大还原糖生成量0.920、1.160、0.929 mg/g,当植酸酶添加水平为1940 U/kg时,获得最大植酸磷降解率92.23%；当4种酶的复合酶谱为β-葡聚糖酶150 U/g、木聚糖酶950 U/g、纤维素酶900 U/g、植酸酶2500 U/kg时,还原糖生成量、植酸磷降解率、饲料残渣总能均获得较优值。综上,肉鸡小麦型饲粮中β-葡聚糖酶、木聚糖酶、纤维素酶和植酸酶的最佳添加水平,1~3周龄分别为150、960、950 U/g和2010 U/kg,4~6周龄分别为150、950、960 U/g和1940 U/kg,且2个阶段肉鸡小麦型饲粮中4种酶的最佳复合酶谱为β-葡聚糖酶150 U/g、木聚糖酶950 U/g、纤维素酶900 U/g、植酸酶2500 U/kg。本試驗旨在研究β-葡聚糖酶、木聚糖酶、纖維素酶和植痠酶用于肉鷄小麥型飼糧的最佳複閤酶譜。採用單因素完全隨機試驗設計,將不同水平的β-葡聚糖酶(30、60、90、120、150和180 U/g)、木聚糖酶(200、400、600、800、1000和1200 U/g)、纖維素酶(200、400、600、800、1000和1200 U/g)和植痠酶(500、1000、1500、2000、2500和3000 U/kg)分彆添加于肉鷄小麥型飼糧中,採用模擬胃腸液體外消化試驗,研究單酶的最佳添加水平；據此,採用4因子3水平L9(34)正交設計和體外法研究4種單酶複配效應,每種酶各設計3箇添加水平：β-葡聚糖酶為100、150和200 U/g,木聚糖酶為900、950和1000 U/g,纖維素酶為900、950和1000 U/g,植痠酶為1500、2000和2500 U/kg,以還原糖生成量、植痠燐降解率、飼料殘渣總能為判定指標,確定4種單酶的最佳複閤酶譜。結果錶明：1~3週齡肉鷄小麥型飼糧中,噹β-葡聚糖酶、木聚糖酶、纖維素酶添加水平分彆為150、960、950 U/g 時,分彆穫得最大還原糖生成量0.918、1.161、0.927 mg/g,噹植痠酶添加水平為2010 U/kg時,穫得最大植痠燐降解率92.35%；4~6週齡肉鷄小麥型飼糧中,噹β-葡聚糖酶、木聚糖酶、纖維素酶添加水平分彆為150、950、960 U/g時,分彆穫得最大還原糖生成量0.920、1.160、0.929 mg/g,噹植痠酶添加水平為1940 U/kg時,穫得最大植痠燐降解率92.23%；噹4種酶的複閤酶譜為β-葡聚糖酶150 U/g、木聚糖酶950 U/g、纖維素酶900 U/g、植痠酶2500 U/kg時,還原糖生成量、植痠燐降解率、飼料殘渣總能均穫得較優值。綜上,肉鷄小麥型飼糧中β-葡聚糖酶、木聚糖酶、纖維素酶和植痠酶的最佳添加水平,1~3週齡分彆為150、960、950 U/g和2010 U/kg,4~6週齡分彆為150、950、960 U/g和1940 U/kg,且2箇階段肉鷄小麥型飼糧中4種酶的最佳複閤酶譜為β-葡聚糖酶150 U/g、木聚糖酶950 U/g、纖維素酶900 U/g、植痠酶2500 U/kg。
본시험지재연구β-포취당매、목취당매、섬유소매화식산매용우육계소맥형사량적최가복합매보。채용단인소완전수궤시험설계,장불동수평적β-포취당매(30、60、90、120、150화180 U/g)、목취당매(200、400、600、800、1000화1200 U/g)、섬유소매(200、400、600、800、1000화1200 U/g)화식산매(500、1000、1500、2000、2500화3000 U/kg)분별첨가우육계소맥형사량중,채용모의위장액체외소화시험,연구단매적최가첨가수평；거차,채용4인자3수평L9(34)정교설계화체외법연구4충단매복배효응,매충매각설계3개첨가수평：β-포취당매위100、150화200 U/g,목취당매위900、950화1000 U/g,섬유소매위900、950화1000 U/g,식산매위1500、2000화2500 U/kg,이환원당생성량、식산린강해솔、사료잔사총능위판정지표,학정4충단매적최가복합매보。결과표명：1~3주령육계소맥형사량중,당β-포취당매、목취당매、섬유소매첨가수평분별위150、960、950 U/g 시,분별획득최대환원당생성량0.918、1.161、0.927 mg/g,당식산매첨가수평위2010 U/kg시,획득최대식산린강해솔92.35%；4~6주령육계소맥형사량중,당β-포취당매、목취당매、섬유소매첨가수평분별위150、950、960 U/g시,분별획득최대환원당생성량0.920、1.160、0.929 mg/g,당식산매첨가수평위1940 U/kg시,획득최대식산린강해솔92.23%；당4충매적복합매보위β-포취당매150 U/g、목취당매950 U/g、섬유소매900 U/g、식산매2500 U/kg시,환원당생성량、식산린강해솔、사료잔사총능균획득교우치。종상,육계소맥형사량중β-포취당매、목취당매、섬유소매화식산매적최가첨가수평,1~3주령분별위150、960、950 U/g화2010 U/kg,4~6주령분별위150、950、960 U/g화1940 U/kg,차2개계단육계소맥형사량중4충매적최가복합매보위β-포취당매150 U/g、목취당매950 U/g、섬유소매900 U/g、식산매2500 U/kg。
The aim of this experiment was to study the optimal compound enzyme preparation containingβ-glu-canase, xylanase, cellulase and phytase added into broiler wheat meal diets. The in vitro digestion tests of broiler wheat meal diets added with different levels of β-glucanase ( 30, 60, 90, 120, 150 and 180 U/g) , xylanase ( 200, 400, 600, 800, 1 000 and 1 200 U/g ) , cellulose ( 200, 400, 600, 800, 1 000 and 1 200 U/g) and phytase (500, 1 000, 1 500, 2 000, 2 500 and 3 000 U/kg) were conducted by one factor complete randomized experiment design to determine the optimal supplemental levels. Then the combination effects of the above four enzymes added in broiler wheat meal diets were evaluated by L9( 34 ) orthogonal de-sign and the in vitro digestion tests, and the supplemental levels were set according to the optimal levels in the first test by β-glucanase (100, 150 and 200 U/g), xylanase (900, 950 and 1 000 U/g), cellulase (900, 950 and 1 000 U/g) and phytase (1 500, 2 000 and 2 500 U/kg). The optimal complex formula of the four enzymes were determined by reducing sugar content, phytate phosphorus degradation rate and the feed residue gross energy. The results showed as follows:when the supplemental levels ofβ-glucanase, xylanase, cellulase were 150, 960 and 950 U/g, respectively, the maximum reducing sugar contents were 0. 918, 1. 161 and 0.927 mg/g, respectively; when the supplemental level of phytase was 2 010 U/kg, the maximum phytate phosphorus degradation rate was 92.35% in 1 to 3-week-old broiler wheat meal diets. When the supplemental levels of β-glucanase, xylanase, cellulase were 150, 950, 960 U/g, the maximum reducing sugar content were 0.920, 1.160, 0.929 mg/g, respectively; when the supplemental level of phytase was 1 940 U/kg, the maximum phytate phosphorus degradation rate was 92.23% in 4 to 6-week-old broiler wheat meal diets. When the compound enzyme preparation containing the above four enzymes was 150 U/gβ-glucanase, 950 U/g xy-lanase, 900 U/g cellulase and 2 500 U/kg phytase in diets, the reducing sugar content, phytate phosphorus degradation rate and feed residue gross energy could all obtain better values. In summary, the optimal supple-mental levels ofβ-glucanase, xylanase, cellulase and phytase are 150, 960, 950 U/g and 2 010 U/kg in 1 to 3-week-old broiler wheat meal diets, respectively, and 150, 950, 960 U/g and 1 940 U/kg in 4 to 6-week-old broiler wheat meal diets, respectively. The optimal compound enzyme preparation containing the above four enzymes is 150 U/g β-glucanase, 950 U/g xylanase, 900 U/g cellulase and 2 500 U/kg phytase in diets.