CAJ | 학술논문

为比较不同外源寡糖对山羊瘤胃液主要酶活及常用粗饲料瘤胃降解率的影响,选用体况良好、体重(62.80±2.45) kg、装有永久性瘤胃瘘管的成年崂山奶山羊去势公羊6只。采用6×4不完全拉丁方试验设计。羊只分别饲喂基础饲粮(对照)及添加1%外源寡糖[甘露寡糖( MOS)、半乳甘露寡糖( GMOS)、寡木糖( XOS)、低聚异麦芽寡糖( IMO)、果寡糖( FOS)]的试验饲粮；试验进行4期,每期35 d,其中预试期25 d,正试期10 d。结果表明：1)饲粮添加MOS可显著提高羧甲基纤维素酶活性( P<0.05)；GMOS、IMO和FOS可显著提高滤纸纤维素酶活性(P<0.05)；添加IMO、XOS、GMOS和FOS可显著或极显著提高β-葡萄糖苷酶活性(P<0.05或P<0.01)；添加MOS、GMOS、XOS、FOS和IMO可显著或极显著提高木聚糖酶活性( P<0.05或P<0.01)；添加IMO、GMOS和FOS可显著提高中性蛋白酶活性( P<0.05)；添加FOS可显著提高α-淀粉酶活性( P<0.05)。2)饲粮添加MOS、GMOS、IMO、XOS、FOS可提高玉米全株青贮、花生蔓、羊草、苜蓿中干物质、中性洗涤纤维、酸性洗涤纤维的瘤胃降解率。综合各项指标,在1%添加水平下,GMOS和FOS在山羊饲粮中添加效果较好。
위비교불동외원과당대산양류위액주요매활급상용조사료류위강해솔적영향,선용체황량호、체중(62.80±2.45) kg、장유영구성류위루관적성년로산내산양거세공양6지。채용6×4불완전랍정방시험설계。양지분별사위기출사량(대조)급첨가1%외원과당[감로과당( MOS)、반유감로과당( GMOS)、과목당( XOS)、저취이맥아과당( IMO)、과과당( FOS)]적시험사량；시험진행4기,매기35 d,기중예시기25 d,정시기10 d。결과표명：1)사량첨가MOS가현저제고최갑기섬유소매활성( P<0.05)；GMOS、IMO화FOS가현저제고려지섬유소매활성(P<0.05)；첨가IMO、XOS、GMOS화FOS가현저혹겁현저제고β-포도당감매활성(P<0.05혹P<0.01)；첨가MOS、GMOS、XOS、FOS화IMO가현저혹겁현저제고목취당매활성( P<0.05혹P<0.01)；첨가IMO、GMOS화FOS가현저제고중성단백매활성( P<0.05)；첨가FOS가현저제고α-정분매활성( P<0.05)。2)사량첨가MOS、GMOS、IMO、XOS、FOS가제고옥미전주청저、화생만、양초、목숙중간물질、중성세조섬유、산성세조섬유적류위강해솔。종합각항지표,재1%첨가수평하,GMOS화FOS재산양사량중첨가효과교호。
This experiment was conducted to study the effects of different oligosaccharides on main enzyme ac-tivities of rumen fluid and degradation rates of roughages in rumen of dairy goats. Six healthy mature castrated Laoshan dairy goats [(62.80±2.45) kg] with permanent cannulas were used. A 6×4 incomplete Latin square design was adopted. Goats were fed a basal diet ( control) and the basal diet supplemented with 1% mannanoli-gosaccharide ( MOS) , galacto-mannanoligosaccharides ( GMOS) , xylooligosaccharide ( XOS) , isomaltooli-gosaccharide ( IMO) and fructooligosaccharide ( FOS) , respectively. The trial lasted for 4 stages with 35 days per stage, and each stage included 25 days pre-test period before 10 days trial period. The results showed as follows:1) compared with the control group, MOS significantly increased the activity of carboxymethyl cellu-lase ( P<0.05) , GMOS, IMO and FOS significantly increased the activity of filter paper cellulase ( P<0.05) , XOS, IMO, FOS and GMOS significantly increased the activity of β-glucosidase ( P<0. 05 or P<0. 01 ) , MOS, GMOS, XOS, FOS and IMO significantly increased the activity of xylanase (P<0.05 or P<0.01), IMO, GMOS and FOS significantly increased the activity of neutral protease ( P<0.05) , and FOS significantly increased the activities of α-amylase (P<0.05). 2) Compared with the control group, MOS, GMOS, IMO XOS and FOS significantly improved the DM, ADF and NDF degradation rates of corn silage, peanut vine, chinensis and alfalfa in rumen. In conclusion, the optimal exogenous oligosaccharides were GMOS and FOS with the supplemental level of 1%.