广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2014年
22期
102-104,158
,共4页
恩拉霉素%杀真菌素链霉菌%紫外%氯化锂%亚硝基胍%复合诱变
恩拉黴素%殺真菌素鏈黴菌%紫外%氯化鋰%亞硝基胍%複閤誘變
은랍매소%살진균소련매균%자외%록화리%아초기고%복합유변
enramycin%Streptomyces fungicidious%UV%LiCl%NTG%combined mutagenesis
从原始生产菌BKSFJ06出发,对其进行了紫外诱变、氯化锂与紫外复合诱变、亚硝基胍诱变和亚硝基胍与紫外的复合诱变及恩拉霉素抗性筛选工作,用HPLC检测方法,以恩拉霉素的产量为指标,筛选高产菌株。最终筛选出的最优突变菌株ERS42-101108产量提高了50%,且该菌株具有良好的遗传稳定性。通过实验内容可以看出,通过亚硝基胍和紫外复合诱变的方法,抗自身产物定向筛选出的突变株具有较优良的性质。
從原始生產菌BKSFJ06齣髮,對其進行瞭紫外誘變、氯化鋰與紫外複閤誘變、亞硝基胍誘變和亞硝基胍與紫外的複閤誘變及恩拉黴素抗性篩選工作,用HPLC檢測方法,以恩拉黴素的產量為指標,篩選高產菌株。最終篩選齣的最優突變菌株ERS42-101108產量提高瞭50%,且該菌株具有良好的遺傳穩定性。通過實驗內容可以看齣,通過亞硝基胍和紫外複閤誘變的方法,抗自身產物定嚮篩選齣的突變株具有較優良的性質。
종원시생산균BKSFJ06출발,대기진행료자외유변、록화리여자외복합유변、아초기고유변화아초기고여자외적복합유변급은랍매소항성사선공작,용HPLC검측방법,이은랍매소적산량위지표,사선고산균주。최종사선출적최우돌변균주ERS42-101108산량제고료50%,차해균주구유량호적유전은정성。통과실험내용가이간출,통과아초기고화자외복합유변적방법,항자신산물정향사선출적돌변주구유교우량적성질。
The original strain BKSFJ06 was treated by UV, UV with lithium chloride, NTG and NTG with UV. The enramycin resistant mutants of Streptomyces fungicidious was isolated on the agar plates containing enramycin. The enramycin resistant mutants could produce high titer of enramycin. The mutant strain ERS42-101108 had good genetic stability and its productivity of enramycin was increased by 50%. The mutation rate of the original strain could be raised greatly by the combined use of NTG and UV that was demonstrated.