CAJ | 학술논문

利用酶辅助法对莲子壳中总黄酮的提取工艺进行优化。在单因子试验结果的基础上,采用响应面法研究了酶用量、酶解时间、 pH值和酶解温度对莲子壳中总黄酮提取得率的影响。结果表明：纤维素酶具有较好的酶解能力,当酶用量为6.5 mg/g,酶解时间为1.5 h, pH 4.7,温度为46℃时,总黄酮得率达到7.1%。
이용매보조법대련자각중총황동적제취공예진행우화。재단인자시험결과적기출상,채용향응면법연구료매용량、매해시간、 pH치화매해온도대련자각중총황동제취득솔적영향。결과표명：섬유소매구유교호적매해능력,당매용량위6.5 mg/g,매해시간위1.5 h, pH 4.7,온도위46℃시,총황동득솔체도7.1%。
The process of enzyme-assisted extraction of total flavonoids from the lotus seed shell was optimized. Based on the single-factor tests, the effects of enzyme amount time of enzymatic hydrolysis, pH value and temperature of enzymatic hydrolysis on the extraction yields of total flavonoids from lotus seed shell were studied by response surface methodology ( RSM) . The results indicated that cellulase showed better ability of enzymatic hydrolysis. The optimum enzyme-assisted extraction process was enzyme amount 6. 5 mg/g, time 1. 5 h, pH value 4. 7 and temperature 46℃. The highest yield of total flavonoids was 7. 1%.