CAJ | 학술논문

目的：建立紫外分光光度法测定半枝莲黄芩苷的含量的方法。方法：采用50%乙醇回流提取工艺和紫外分光光度法测定半枝莲中黄芩苷的含量，以黄芩苷为对照品，在278nm波长处测量。结果：得黄芩苷回归方程y=56.985x+0.0083(r2=0.9996)，表明黄芩苷在0.00272~0.0136mg/mL浓度范围内线性关系良好，平均加样回收率(n=6)以黄芩苷计为99.8%，RSD为0.58%。结论：此方法操作简单，精密度、稳定性和重复性良好，可以用于半枝莲中黄芩苷的含量测定方法之一。
목적：건립자외분광광도법측정반지련황금감적함량적방법。방법：채용50%을순회류제취공예화자외분광광도법측정반지련중황금감적함량，이황금감위대조품，재278nm파장처측량。결과：득황금감회귀방정y=56.985x+0.0083(r2=0.9996)，표명황금감재0.00272~0.0136mg/mL농도범위내선성관계량호，평균가양회수솔(n=6)이황금감계위99.8%，RSD위0.58%。결론：차방법조작간단，정밀도、은정성화중복성량호，가이용우반지련중황금감적함량측정방법지일。
[Abstract]Objective: Establish UV spectrophotometry banzhilian baicalin content method. Methods: With 50% ethanol extraction process and ultraviolet spectrophotometry banzhilian content baicalin, baicalin as standard, measured at 278nm wavelength. Results: Baicalin regression equation was y=56.985x+0.083 (r2=0.9996), indicates that baicalin in the concentration range of 0.00272~0.0136mg/mL good linear relationship, the average recovery (n=6) to 99.8 meter baicalin , RSD was 0.58%. Conclusion: This method is simple, precision, stability and good reproducibility can be used banzhilian baicalin one method for the determination of the content.