CAJ | 학술논문

糖组学研究通常包括聚糖组的分离与纯化、糖链组的分离和富集糖链的结构解析和定量以及糖链的性质和功能研究。根据糖蛋白组、蛋白聚糖组和糖脂组生物化学性质的不同，可相应采用分步沉淀法、硼酸亲和法、二氧化钛法、亲和层析法、体积排阻法、凝胶过滤层析和柱层析法等进行分离与纯化，进而通过植物凝集素、亲水色谱和固相萃取等技术富集高纯度且特异性的糖链。通过凝集素芯片技术、各种生物质谱及其联用并辅以糖链的衍生化标记对糖链进行结构解析，并用同位素标记法和代谢标记法对糖链进行相对定量。最后，借助糖链结构解析软件工具及糖链相关数据库进行生物信息学分析，可对糖链的生物学功能进行更全面的解析。本文对复合糖的分离纯化、结构分析以及糖组生物信息研究方法进行了综述。
당조학연구통상포괄취당조적분리여순화、당련조적분리화부집당련적결구해석화정량이급당련적성질화공능연구。근거당단백조、단백취당조화당지조생물화학성질적불동，가상응채용분보침정법、붕산친화법、이양화태법、친화층석법、체적배조법、응효과려층석화주층석법등진행분리여순화，진이통과식물응집소、친수색보화고상췌취등기술부집고순도차특이성적당련。통과응집소심편기술、각충생물질보급기련용병보이당련적연생화표기대당련진행결구해석，병용동위소표기법화대사표기법대당련진행상대정량。최후，차조당련결구해석연건공구급당련상관수거고진행생물신식학분석，가대당련적생물학공능진행경전면적해석。본문대복합당적분리순화、결구분석이급당조생물신식연구방법진행료종술。
Technologies for glycomics usually involve methods for separation and purification of poly-saccharides, and separation, structure resolution, quantification, property investigation and function comment of glycan chains. Because of the different biochemical properties of glycoproteins, proteogly-cans and glycolipids, the separation and purification of polysaccharides involve corresponding fractional precipitation, boric acid affinity, titanium dioxide, affinity chromatography, size exclusion method, and gel filtration chromatography column chromatography methods. The lectins, water affinity chromatogra-phy , solid phase extraction and other technologies could be applied to the oil enrichment of high pure and specific glycan chains. The structure of glycan chains can be analyzed using lectin microarray technolo-gy, mass spectrometry, and derivatization markers of glycan chains. lsotope labelling and metabolic labeling can be used to quantify glycan chains. The glycan biological function can be better understood using glycan chain structure analysis software and database of glycan chains by bioinformatics.