中国药理学与毒理学杂志
中國藥理學與毒理學雜誌
중국약이학여독이학잡지
CHINESE JOURNAL OF PHARMACOLOGY AND TOXICOLOGY
2014年
6期
857-862
,共6页
郜娜%高远%田锋奇%乔海灵
郜娜%高遠%田鋒奇%喬海靈
고나%고원%전봉기%교해령
黄芩苷%药物过敏%抗体生成
黃芩苷%藥物過敏%抗體生成
황금감%약물과민%항체생성
baicalin%drug hypersensitivity%antibody formation
目的：探讨黄芩苷的致敏作用机制。方法采用活性酯法制备黄芩苷全抗原，建立黄芩苷致豚鼠过敏性休克模型；采用被动皮肤过敏反应( PCA)实验分析豚鼠特异性抗体产生的规律性以及lgE和lgG抗体效价，采用ELlSA测定豚鼠血清中黄芩苷特异性lgG抗体。结果成功合成黄芩苷全抗原，黄芩苷与牛血清蛋白( BSA)和人血清白蛋白( HSA)的偶联效率分别为9∶1和5∶1。成功建立黄芩苷致敏的豚鼠休克模型，并显示典型的速发型过敏反应特征。致敏豚鼠第一次致敏后第19天开始用PCA实验检测出抗黄芩苷抗体，第31天达高峰，之后逐渐降低。致敏豚鼠体内同时存在lgE和lgG型特异性抗体，且两抗体之间无明显相关性。致敏豚鼠血清中黄芩苷特异性lgG抗体的阳性率为84％，致敏组肥大细胞脱颗粒率为(72.6±11.4)％，显著高于阴性对照组(26.8±6.9)％。致敏豚鼠离体回肠经黄芩苷-HSA激发后收缩明显。结论黄芩苷能够导致豚鼠出现过敏反应，并与特异性lgE和lgG抗体有关。
目的：探討黃芩苷的緻敏作用機製。方法採用活性酯法製備黃芩苷全抗原，建立黃芩苷緻豚鼠過敏性休剋模型；採用被動皮膚過敏反應( PCA)實驗分析豚鼠特異性抗體產生的規律性以及lgE和lgG抗體效價，採用ELlSA測定豚鼠血清中黃芩苷特異性lgG抗體。結果成功閤成黃芩苷全抗原，黃芩苷與牛血清蛋白( BSA)和人血清白蛋白( HSA)的偶聯效率分彆為9∶1和5∶1。成功建立黃芩苷緻敏的豚鼠休剋模型，併顯示典型的速髮型過敏反應特徵。緻敏豚鼠第一次緻敏後第19天開始用PCA實驗檢測齣抗黃芩苷抗體，第31天達高峰，之後逐漸降低。緻敏豚鼠體內同時存在lgE和lgG型特異性抗體，且兩抗體之間無明顯相關性。緻敏豚鼠血清中黃芩苷特異性lgG抗體的暘性率為84％，緻敏組肥大細胞脫顆粒率為(72.6±11.4)％，顯著高于陰性對照組(26.8±6.9)％。緻敏豚鼠離體迴腸經黃芩苷-HSA激髮後收縮明顯。結論黃芩苷能夠導緻豚鼠齣現過敏反應，併與特異性lgE和lgG抗體有關。
목적：탐토황금감적치민작용궤제。방법채용활성지법제비황금감전항원，건립황금감치돈서과민성휴극모형；채용피동피부과민반응( PCA)실험분석돈서특이성항체산생적규률성이급lgE화lgG항체효개，채용ELlSA측정돈서혈청중황금감특이성lgG항체。결과성공합성황금감전항원，황금감여우혈청단백( BSA)화인혈청백단백( HSA)적우련효솔분별위9∶1화5∶1。성공건립황금감치민적돈서휴극모형，병현시전형적속발형과민반응특정。치민돈서제일차치민후제19천개시용PCA실험검측출항황금감항체，제31천체고봉，지후축점강저。치민돈서체내동시존재lgE화lgG형특이성항체，차량항체지간무명현상관성。치민돈서혈청중황금감특이성lgG항체적양성솔위84％，치민조비대세포탈과립솔위(72.6±11.4)％，현저고우음성대조조(26.8±6.9)％。치민돈서리체회장경황금감-HSA격발후수축명현。결론황금감능구도치돈서출현과민반응，병여특이성lgE화lgG항체유관。
OBJECTlVE To elucidate the sensitization mechanism of baicalin in guinea pigs. METHODS Baicalin antigens were prepared by activated ester method. A guinea pig model of allergic shock induced by the coupling was established. Passive cutaneous anaphylaxis ( PCA) was used to ana-lyze the regularity of the specific antibody. The lgG antibodies of baicalin in serum among 19 sensitized guinea pigs were determined by ELlSA. RESULTS UV scanning spectrum couplings occurred between baicalin and protein ( bovine serum albumin and human serum albumin) . The conjugation ratio was 9∶1 and 5∶1, respectively. The guinea pig model of allergic shock was successfully established and suggested that the antigen induced a typical immediate hypersensitivity response. The antibody could be determined on the 19th day and reached the maximum on the 31st day and then degraded gradually. Both specific lgE and lgG existed in the sensitized serum, but there was no correlation between lgG and lgE. The pos-itive rate of specific lgG antibody to baicalin was 84%. The degranulation rate of peritoneal mast cells was (72.6±11.4)%and higher than that incubated with plasma from control (26.8±6.9)%(P<0.05). lsolated ileum from sensitized guinea pigs contracted conspicuously after being challenged by baicalin-human serum albumin. CONCLUSlON Baicalin can induce allergic reaction in guinea pigs and the reaction is correlated with lgE and lgG production.
