CAJ | 학술논문

目的：应用小鼠显性致死试验评价西番莲果汁的遗传毒性。方法：西番莲果汁7.2～28.8 g/kg剂量对NIH种雄性小鼠连续灌胃染毒5 d，再与雌性小鼠连续同笼交配7周，每批雌性小鼠在妊娠第13～14天处死，观察胚胎着床数、活胎数和死胎数。同时设置对照组。对所有计数、计量资料进行统计学处理。结果：环磷酰胺(CP)和丝裂霉素C(MMC)阳性对照小鼠除平均着床数正常外，其余各项指标在不同周次中均存在异常(P<0.05或<0.01)。阴性对照组和西番莲果汁各剂量组的小鼠交配率>133%，妊娠率在60%～93%，窝平均着床数>8.6只，平均死胎数<0.8只，平均活胎数>8.1只，均在正常数值范围内；各剂量西番莲果汁均未诱发有死胎孕鼠率、显性致死率和突变指数的增高及着床卵生存率的降低(P>0.05)。结论：西番莲果汁在本试验剂量(7.2～28.8 g/kg)范围内，小鼠显性致死试验为阴性结果，提示其无遗传毒性。
목적：응용소서현성치사시험평개서번련과즙적유전독성。방법：서번련과즙7.2～28.8 g/kg제량대NIH충웅성소서련속관위염독5 d，재여자성소서련속동롱교배7주，매비자성소서재임신제13～14천처사，관찰배태착상수、활태수화사태수。동시설치대조조。대소유계수、계량자료진행통계학처리。결과：배린선알(CP)화사렬매소C(MMC)양성대조소서제평균착상수정상외，기여각항지표재불동주차중균존재이상(P<0.05혹<0.01)。음성대조조화서번련과즙각제량조적소서교배솔>133%，임신솔재60%～93%，와평균착상수>8.6지，평균사태수<0.8지，평균활태수>8.1지，균재정상수치범위내；각제량서번련과즙균미유발유사태잉서솔、현성치사솔화돌변지수적증고급착상란생존솔적강저(P>0.05)。결론：서번련과즙재본시험제량(7.2～28.8 g/kg)범위내，소서현성치사시험위음성결과，제시기무유전독성。
OBJECTIVE:In order to evaluate the genetic toxicity of passiflora fruit juice,the mice dominant lethal test was conducted. METHODS:Male NIH mice were gavaged for 5 consecutive days with 7.2-28.8 g/kg passiflora fruit juice,with positive and negative controls. Then individual males were mated sequentially at predetermined intervals th thwith two virgin females. Females were sacrificed on the 13 -14 day of pregnancy and the number of embryo implantated,live births and stillbirths were recorded. All data were evaluated by appropriate statistical methods. RESULTS:For cyclophosphamide and mitomycin C groups,all indicators in different weeks were abnormal in addition to the number of nidation(P<0.05 or P<0.01). For negative and treated groups,mating rate (>133%),pregnancy rate (60%-93%),number of nidation (>8.6),average number of stillbirths (<0.8) and live births (>8.1) were all in the normal ranges. There were no significant increase in stillbirth rate,dominant lethality and the mutation index nor reduction in fertilized survival of eggs (P>0.05). CONCLUSION:7.2-28.8 g/kg passiflora fruit juice did not show a positive response in mice dominant lethal test,indicating that the possibility of genetic toxicity was small.