CAJ | 학술논문

目的：探讨与肿瘤生长浸润和转移相关的表皮生长因子受体(HER-2)、基质金属蛋白酶-2(MMP-2)、胰岛素样生长因子-1(IGF-1)和血管内皮生长因子(VEGF)在新疆维吾尔族妇女宫颈癌组织中的表达及意义。方法：收集维吾尔族妇女浸润性宫颈鳞癌患者的宫颈癌组织38例，并收集正常宫颈组织32例作为正常对照。分别运用实时荧光定量PCR(Q-PCR)和蛋白免疫印迹(Western blot)技术检测HER-2、MMP-2、IGF-1和VEGF在不同宫颈组织中的mRNA及蛋白表达水平。结果：Q-PCR检测结果显示，与正常对照组相比， HER-2、IGF-1、MMP-2和VEGF的mRNA表达水平在宫颈组织中均有不同程度的升高，差异均有统计学意义(P<0.05或<0.01)。Western blot检测显示HER-2、MMP-2和VEGF蛋白表达水平在宫颈癌组中的表达亦均较正常对照组有不同程度的升高，差异均有统计学意义(P<0.05或P<0.01)，而IGF-1在两组之间的表达差异无统计学意义(P>0.05)。结论：HER-2、MMP-2、IGF-1和VEGF在维吾尔族妇女宫颈癌组织中的过度表达可能与宫颈癌的发生密切相关。
목적：탐토여종류생장침윤화전이상관적표피생장인자수체(HER-2)、기질금속단백매-2(MMP-2)、이도소양생장인자-1(IGF-1)화혈관내피생장인자(VEGF)재신강유오이족부녀궁경암조직중적표체급의의。방법：수집유오이족부녀침윤성궁경린암환자적궁경암조직38례，병수집정상궁경조직32례작위정상대조。분별운용실시형광정량PCR(Q-PCR)화단백면역인적(Western blot)기술검측HER-2、MMP-2、IGF-1화VEGF재불동궁경조직중적mRNA급단백표체수평。결과：Q-PCR검측결과현시，여정상대조조상비， HER-2、IGF-1、MMP-2화VEGF적mRNA표체수평재궁경조직중균유불동정도적승고，차이균유통계학의의(P<0.05혹<0.01)。Western blot검측현시HER-2、MMP-2화VEGF단백표체수평재궁경암조중적표체역균교정상대조조유불동정도적승고，차이균유통계학의의(P<0.05혹P<0.01)，이IGF-1재량조지간적표체차이무통계학의의(P>0.05)。결론：HER-2、MMP-2、IGF-1화VEGF재유오이족부녀궁경암조직중적과도표체가능여궁경암적발생밀절상관。
OBJECTIVE:To investigate the expression of HER-2,MMP-2,IGF-1 and VEGF with cervical cancer for clinical assessment. METHODS:We collected cervical tissue samples from Uygur women with cervical lesions (invasive cervical cancer,ICC:38 cases,cervicitis:32 cases). The mRNA and protein expression levels of HER-2,MMP-2,IGF-1and VEGF in tissues from two groups were examined by real-time fluorescence quantitative PCR (Q-PCR) and Western blot. RESULTS:Compared with normal control group,the expressions of HER-2,MMP-2,IGF-1 and VEGF in ICC group were increased to different extent at mRNA level,and the difference was statistically significant (P<0.05 or P<0.01). At protein level,the expressions of HER-2,MMP-2 and VEGF in ICC group were increased to different degree,and the difference was statistically significant (P<0.05 or P<0.01),but there was no statistically significant difference in IGF-1 expression (P>0.05). CONCLUSION:The excessive expressions of HER-2,MMP-2,IGF-1 and VEGF may be related to the occurrence and development of cervical cancer.