癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2014年
6期
441-445
,共5页
周庆红%姜淑卿%刘英华%张力%赵春红%王晓军%张大龙%张静姝
週慶紅%薑淑卿%劉英華%張力%趙春紅%王曉軍%張大龍%張靜姝
주경홍%강숙경%류영화%장력%조춘홍%왕효군%장대룡%장정주
纳米硫化镉%精子畸形%睾酮%细胞色素P450%胆固醇侧链裂解酶%类固醇17-α-羟化酶
納米硫化鎘%精子畸形%睪酮%細胞色素P450%膽固醇側鏈裂解酶%類固醇17-α-羥化酶
납미류화력%정자기형%고동%세포색소P450%담고순측련렬해매%류고순17-α-간화매
cadmiumsulfidenano-particles%sperm abnormality%testosterone%cytochrome P450%side-chain cleavage enzyme%steroid 17-alpha-hydroxylase
目的：探讨纳米硫化镉(Nano-CdS)和硫化镉(CdS)的雄性生殖毒性作用机制并比较其生殖毒性效应。方法：将36只SPF级雄性ICR小鼠随机分为对照组、Nano-CdS组和CdS组，每组各12只。两个染毒组经口灌胃染毒，每天灌胃1次，染毒剂量均为50 mg/kg，对照组给予等体积的生理盐水，连续45 d。采用石墨炉原子吸收光谱法检测小鼠睾丸组织中镉元素积累水平，显微镜下观察小鼠精子畸形率，采用酶联免疫吸附法(ELISA)检测小鼠血清睾酮水平，采用荧光实时定量PCR检测P450scc和P450-17αmRNA表达水平。结果：镉元素含量分析结果显示， Nano-CdS和CdS组小鼠睾丸组织中的镉元素含量分别为(425.46±73.89)和(183.59±32.46) ng/g，均高于对照组的(80.18±13.29) ng/g (P<0.05)。精子畸形率检测结果显示，CdS组小鼠(2.28%)显著高于对照组(1.48%)(P<0.05)，Nano-CdS组(1.73%)也有所升高。ELISA检测结果显示， Nano-CdS和CdS组小鼠血清睾酮浓度分别为(12.31±1.10)和(15.88±5.41)ng/dL，均明显低于对照组的(68.41±11.36) ng/dL(P<0.05)。荧光实时定量PCR检测结果显示，Nano-CdS和CdS组P450scc mRNA的表达水平分别为0.50±0.11和0.84±0.48；P450-17α mRNA的表达水平分别为0.51±0.13和0.72±0.06，除CdS组P450scc mRNA表达水平外，其他各组均明显低于对照组(P<0.05)。结论：Nano-CdS和CdS均能导致小鼠睾丸中镉元素的积累，一定程度上诱导精子畸形率的升高，并能显著降低血清睾酮水平以及抑制P450scc和P450-17αmRNA的表达水平。
目的：探討納米硫化鎘(Nano-CdS)和硫化鎘(CdS)的雄性生殖毒性作用機製併比較其生殖毒性效應。方法：將36隻SPF級雄性ICR小鼠隨機分為對照組、Nano-CdS組和CdS組，每組各12隻。兩箇染毒組經口灌胃染毒，每天灌胃1次，染毒劑量均為50 mg/kg，對照組給予等體積的生理鹽水，連續45 d。採用石墨爐原子吸收光譜法檢測小鼠睪汍組織中鎘元素積纍水平，顯微鏡下觀察小鼠精子畸形率，採用酶聯免疫吸附法(ELISA)檢測小鼠血清睪酮水平，採用熒光實時定量PCR檢測P450scc和P450-17αmRNA錶達水平。結果：鎘元素含量分析結果顯示， Nano-CdS和CdS組小鼠睪汍組織中的鎘元素含量分彆為(425.46±73.89)和(183.59±32.46) ng/g，均高于對照組的(80.18±13.29) ng/g (P<0.05)。精子畸形率檢測結果顯示，CdS組小鼠(2.28%)顯著高于對照組(1.48%)(P<0.05)，Nano-CdS組(1.73%)也有所升高。ELISA檢測結果顯示， Nano-CdS和CdS組小鼠血清睪酮濃度分彆為(12.31±1.10)和(15.88±5.41)ng/dL，均明顯低于對照組的(68.41±11.36) ng/dL(P<0.05)。熒光實時定量PCR檢測結果顯示，Nano-CdS和CdS組P450scc mRNA的錶達水平分彆為0.50±0.11和0.84±0.48；P450-17α mRNA的錶達水平分彆為0.51±0.13和0.72±0.06，除CdS組P450scc mRNA錶達水平外，其他各組均明顯低于對照組(P<0.05)。結論：Nano-CdS和CdS均能導緻小鼠睪汍中鎘元素的積纍，一定程度上誘導精子畸形率的升高，併能顯著降低血清睪酮水平以及抑製P450scc和P450-17αmRNA的錶達水平。
목적：탐토납미류화력(Nano-CdS)화류화력(CdS)적웅성생식독성작용궤제병비교기생식독성효응。방법：장36지SPF급웅성ICR소서수궤분위대조조、Nano-CdS조화CdS조，매조각12지。량개염독조경구관위염독，매천관위1차，염독제량균위50 mg/kg，대조조급여등체적적생리염수，련속45 d。채용석묵로원자흡수광보법검측소서고환조직중력원소적루수평，현미경하관찰소서정자기형솔，채용매련면역흡부법(ELISA)검측소서혈청고동수평，채용형광실시정량PCR검측P450scc화P450-17αmRNA표체수평。결과：력원소함량분석결과현시， Nano-CdS화CdS조소서고환조직중적력원소함량분별위(425.46±73.89)화(183.59±32.46) ng/g，균고우대조조적(80.18±13.29) ng/g (P<0.05)。정자기형솔검측결과현시，CdS조소서(2.28%)현저고우대조조(1.48%)(P<0.05)，Nano-CdS조(1.73%)야유소승고。ELISA검측결과현시， Nano-CdS화CdS조소서혈청고동농도분별위(12.31±1.10)화(15.88±5.41)ng/dL，균명현저우대조조적(68.41±11.36) ng/dL(P<0.05)。형광실시정량PCR검측결과현시，Nano-CdS화CdS조P450scc mRNA적표체수평분별위0.50±0.11화0.84±0.48；P450-17α mRNA적표체수평분별위0.51±0.13화0.72±0.06，제CdS조P450scc mRNA표체수평외，기타각조균명현저우대조조(P<0.05)。결론：Nano-CdS화CdS균능도치소서고환중력원소적적루，일정정도상유도정자기형솔적승고，병능현저강저혈청고동수평이급억제P450scc화P450-17αmRNA적표체수평。
OBJECTIVE:To compare the effects of normal cadmium sulfide (CdS) and Nano-CdS on the male reproductive system of mice,and to investigate the mechanisms of reproductive toxicity. METHODS:36 male ICR mice were randomly divided into control group,and two experimental groups which were exposed to 50 mg/kg Nano-CdS and CdS,respectively. Mice in control group were exposed to same volume of physiological saline . After 45 days,levels of cadmium accumulation in testis were determined directly by GF AAS. The rate of sperm abnormality was also calculated. Serum testosterone levels were measured by enzyme-linked immunosorbent assay (ELISA),expression levels of P450scc and P450-17α mRNA were determined by real-time PCR. RESULTS:Contents of cadmium in Nano-CdS and CdS groups were (425.46±73.89) and (183.59±32.46) ng/g,respectively,and were higher than the control (80.18±13.29) (P<0.05). Sperm deformity rate in CdS group (2.28%) was significantly higher than the control (1.48%) (P<0.05),Nano-CdS group (1.73%) was also increased. Concentrations of testosterone in CdS and Nano-CdS groups were(12.31±1.10) and (15.88±5.41) ng/dL,respectively,and were significantly lower than control group (68.41±11.36) ng/dL (P<0.05). Expression levels of P450scc mRNA in Nano-CdS and CdS groups were (0.50±0.11) and (0.84±0.48),respectively, expression levels of P450-17αmRNA were (0.51±0.13) and (0.72±0.06),respectively. Except P450scc mRNA in CdS group,the mRNA expression levels in experimental groups were significantly lower than control group (P<0.05). CONCLUSION:Content of cadmium accumulation in testis and sperm deformity rate were increased by exposure to Nano-CdS and CdS,with a significant reduction in serum testosterone levels and expression levels of P450scc and P450-17αmRNA.
