CAJ | 학술논문

目的：探讨曲酸对受辐射中国仓鼠卵巢细胞(CHO)的保护作用。方法：CHO细胞分别经不同强度(0~20 Gy ) Coγ射线照射，于照射后24、48和72 h采用MTT法检测细胞存活情况；细胞经不同浓度(分别为0、0.01、0.1、1、10、100μg/mL)曲酸作用1.5 h，采用12 Gyγ射线照射，于照射后24、48和72 h采用MTT法检测细胞存活情况；细胞经浓度为1μg/mL的曲酸作用1.5 h，分别经不同强度(6、12、16 Gy)γ射线照射，于照射后24 h采用MTT法检测细胞存活情况。结果：与对照组比较，γ射线照射导致CHO细胞的存活率下降(P均<0.01)，且随着照射强度的增加，该效应增强；曲酸在0.01～10μg/mL浓度范围内能明显提高12 Gy辐射条件下CHO细胞的存活率，其中浓度在1μg/mL时效应最明显；1μg/mL曲酸均能明显提高6、12、16 Gyγ射线照射下细胞的存活率。结论：曲酸明显提高受辐射CHO细胞的存活率，以保护细胞免受辐射损伤。
목적：탐토곡산대수복사중국창서란소세포(CHO)적보호작용。방법：CHO세포분별경불동강도(0~20 Gy ) Coγ사선조사，우조사후24、48화72 h채용MTT법검측세포존활정황；세포경불동농도(분별위0、0.01、0.1、1、10、100μg/mL)곡산작용1.5 h，채용12 Gyγ사선조사，우조사후24、48화72 h채용MTT법검측세포존활정황；세포경농도위1μg/mL적곡산작용1.5 h，분별경불동강도(6、12、16 Gy)γ사선조사，우조사후24 h채용MTT법검측세포존활정황。결과：여대조조비교，γ사선조사도치CHO세포적존활솔하강(P균<0.01)，차수착조사강도적증가，해효응증강；곡산재0.01～10μg/mL농도범위내능명현제고12 Gy복사조건하CHO세포적존활솔，기중농도재1μg/mL시효응최명현；1μg/mL곡산균능명현제고6、12、16 Gyγ사선조사하세포적존활솔。결론：곡산명현제고수복사CHO세포적존활솔，이보호세포면수복사손상。
OBJECTIVE: To explore the protection of kojic acid (KA) on CHO cells from radiation. METHODS:MTT assay was used to detect cell viability in the following conditions:CHO cells were exposed to different 60intensities (0-20 Gy) of Coγray,and were cultured for 24,48 and 72 h. CHO cells were treated with KA (0、0.01、0.1、1、10、100μg/mL,respectively) for 1.5 h before exposure to 12 Gyγrays,and were cultured for 24,48 and 72 h,respectively;CHO cells were treated with KA at 1μg/mL for 1.5 h prior to exposed to 6,12 and 16 Gyγray,then were cultured for 24 h. RESULTS:CHO cell viability was decreased when they were exposed toγray in a dose-response manner. Cell viability was significantly increased when treated with 0.01-10 μg/mL KA before exposure to 12 Gy γray,with a peak effect at 1 μg/mL. Pretreatment of 1 μg/mL KA could significantly improve the viability of CHO cells exposed to 6,12,16 Gyγrays. CONCLUSION:Pretreatment with KA significantly improved the viability of irradiated CHO cells by its protective effect from radiation damage.