中国药物警戒
中國藥物警戒
중국약물경계
CHINESE JOURNAL OF PHARMACOVIGILANCE
2014年
11期
661-663
,共3页
许伟英%郑新颖%谷晓颖%任世禾
許偉英%鄭新穎%穀曉穎%任世禾
허위영%정신영%곡효영%임세화
高效液相色谱法(HPLC)%复方生地合剂%绿原酸
高效液相色譜法(HPLC)%複方生地閤劑%綠原痠
고효액상색보법(HPLC)%복방생지합제%록원산
high pressure liquid chromatography(HPLC)%Fufang Shengdi mixture%chlorogenic acid
目的:建立复方生地合剂中绿原酸含量测定的高效液相色谱法。方法色谱柱(250 mm×4.6 mm,5μm),流动相:乙腈-0.4%磷酸溶液(10:90),流速1 mL·min-1,柱温30℃,检测波长327 nm。结果绿原酸在0.0624~4.68μg范围内线性关系良好。R=0.9999,平均回收率为100.7%,RSD=1.7%(n=6)。结论该方法操作简便,重复性高,可用于复方生地合剂的质量控制。
目的:建立複方生地閤劑中綠原痠含量測定的高效液相色譜法。方法色譜柱(250 mm×4.6 mm,5μm),流動相:乙腈-0.4%燐痠溶液(10:90),流速1 mL·min-1,柱溫30℃,檢測波長327 nm。結果綠原痠在0.0624~4.68μg範圍內線性關繫良好。R=0.9999,平均迴收率為100.7%,RSD=1.7%(n=6)。結論該方法操作簡便,重複性高,可用于複方生地閤劑的質量控製。
목적:건립복방생지합제중록원산함량측정적고효액상색보법。방법색보주(250 mm×4.6 mm,5μm),류동상:을정-0.4%린산용액(10:90),류속1 mL·min-1,주온30℃,검측파장327 nm。결과록원산재0.0624~4.68μg범위내선성관계량호。R=0.9999,평균회수솔위100.7%,RSD=1.7%(n=6)。결론해방법조작간편,중복성고,가용우복방생지합제적질량공제。
Objective To establish a HPLC method for the determination of chlorogenic acid in Fufang Shengdi mixture. Methods A SHISEIDO MG C18 column(250 mm×4.6 mm, 5μm) was used with the mobile phase of 0.4%phosphoric acid and acetonitrile for isocratic elution (10:90) at the wavelength of 327 nm, with the flow rate of 1mL·min-1 and the column temperature was 30℃. Results The calibration curves for chlorogenic acid were linear in the concentration ranges of 0.0624~4.68μg, and the correlation coefficient was 0.999999. The average recovery was 100.7%,(RSD=1.7%, n=6). Conclusion This method is simple and reproducible,and is suitable for the quality control of the Fufang Shengdi mixture.