CAJ | 학술논문

目的：在小鼠全脑缺血再灌注损伤模型的基础上，根据芎汤加味方所含物质基础研究进展，进行其提取工艺研究。方法结合文献进行芎汤加味方6个制备工艺的研究，测定各工艺提取物中有效成分的含量，并观察各个提取物对小鼠全脑缺血再灌注损伤模型的保护作用。通过物质基础的含量测定和抗脑血活性的比较，筛选最有效的提取工艺。结果通过挥发油、核桃油、阿魏酸提取率指标进行综合评价，工艺5提取物的有效成分保留相对全面，对小鼠全脑缺血和缺氧损伤程度的改善作用最强。结论采用“质量-药效”综合评价模式可保障中药复方的有效性。初步确定了工艺5为芎汤加味方的最佳提取工艺，其具体优化参数有待于进一步研究。
목적：재소서전뇌결혈재관주손상모형적기출상，근거궁탕가미방소함물질기출연구진전，진행기제취공예연구。방법결합문헌진행궁탕가미방6개제비공예적연구，측정각공예제취물중유효성분적함량，병관찰각개제취물대소서전뇌결혈재관주손상모형적보호작용。통과물질기출적함량측정화항뇌혈활성적비교，사선최유효적제취공예。결과통과휘발유、핵도유、아위산제취솔지표진행종합평개，공예5제취물적유효성분보류상대전면，대소서전뇌결혈화결양손상정도적개선작용최강。결론채용“질량-약효”종합평개모식가보장중약복방적유효성。초보학정료공예5위궁탕가미방적최가제취공예，기구체우화삼수유대우진일보연구。
Objective Extraction process of modified Xiongqiong decoction was researched and optimized on the basis of mice models of cerebral ischemia-reperfusion damages according to its material basis. Methods Six extraction processes of modified Xiongqiong decoction were explored according to modern references. Contents of active ingredients in the extracts were determined and protective effects of them on mice models of cerebral ischemia-reperfusion damages were observed. Optimum extraction process was selected according to determination of material basis and comparison of anti-cerebral ischemia. Results Active ingredients in the extract of the fifth extraction process is comprehensive relatively according to the integrated evaluation to volatile oil, walnut oil and ferulic acid. Extract of the fifth extraction process improved most obviously cerebral ischemia-reperfusion damages in all the extracts. Conclusion Model of"quality-efficacy" comprehensive assessment can guarantee effectiveness of Chinese medicine compound. The fifth extraction process has been confirmed to be optimum extraction process of modified Xiongqiong decoction preliminarily. However specific optimum parameters of the fifth extraction process need to be confirmed by a further study.