CAJ | 학술논문

以N-羟基琥珀酰亚胺己二酸酯（NHS-AA）为交联剂,交联改性碱溶胶原,采用二维红外相关光谱法研究了交联对胶原二级结构的影响。研究发现,交联未影响胶原红外特征吸收峰的位置,但1672、1554和1241cm-1归属于胶原酰胺I带的CO伸缩振动、酰胺Ⅱ带的C—N伸缩与N—H弯曲振动和Ⅲ带的N—H面内变形振动峰之间存在同步正交叉峰,表明随交联共价键的增加,胶原的链段构象发生了变化。在NHS-AA用量增加的过程中,胶原二级结构变化的顺序为：酰胺Ⅲ带〉酰胺Ⅰ带〉酰胺Ⅱ带〉—CH3〉—CH—。由此可见,二维红外相关分析法能提供由交联引起的胶原构象动态变化的微观信息,为进一步研究改性胶原结构与功能之间的关系提供实验依据。
이N-간기호박선아알기이산지（NHS-AA）위교련제,교련개성감용효원,채용이유홍외상관광보법연구료교련대효원이급결구적영향。연구발현,교련미영향효원홍외특정흡수봉적위치,단1672、1554화1241cm-1귀속우효원선알I대적CO신축진동、선알Ⅱ대적C—N신축여N—H만곡진동화Ⅲ대적N—H면내변형진동봉지간존재동보정교차봉,표명수교련공개건적증가,효원적련단구상발생료변화。재NHS-AA용량증가적과정중,효원이급결구변화적순서위：선알Ⅲ대〉선알Ⅰ대〉선알Ⅱ대〉—CH3〉—CH—。유차가견,이유홍외상관분석법능제공유교련인기적효원구상동태변화적미관신식,위진일보연구개성효원결구여공능지간적관계제공실험의거。
Alkali-solubilized collagen solution cross-linked with N-hydroxysuccinimide activated adipic acid（NHS-AA） ester.The effect of NHS-AA on the secondary structure of alkali-solubilized collagen was examined.The infrared bands,determined by the Fourier-transform infrared spectroscopy（FT-IR）,did not reflect characteristics which were caused the amount of cross-linking,but the results from two-dimensional infrared correlation spectroscopy（2D-IR） indicated that the positive synchronous cross-peaks,derived from stretching vibrations of C=O at 1672cm-1,C—N stretching vibrations and wagging of N—H at 1554cm-1 and in plane deformation of N—H at 1241cm-1 of collagen,were indicative of local conformational changes of collagen with increasing cross-linking.The order of secondary structure changes of cross-linked collagen was amide Ⅲ than amide Ⅰ than amide Ⅱ than —CH3 than —CH—.It was shown that the microcosmic information of a change in dynamic structure could be provided for cross-linked collagen with the interruption of the cross-linker concentration.These fundamental data should provide available information for understanding of the relationship between the structure and function of cross-linked collagen.