CAJ | 학술논문

采用电沉积法在玻碳（GC）电极表面合成纳米级聚吡咯（PPy）,通过扫描电镜得到PPy的形貌。以PPy为载体,通过吸附法固定葡萄糖氧化酶（GOD）,得到GOD/PPy/GC电极。利用循环伏安法对GOD/PPy/GC电极的电化学行为进行分析,结果表明,以PPy为载体可以很好地固定GOD并保持其生物活性。在0.1mol/L磷酸盐缓冲溶液中,无任何电子媒介体存在时,GOD/PPy/GC电极显示了很好的电催化性能。
채용전침적법재파탄（GC）전겁표면합성납미급취필각（PPy）,통과소묘전경득도PPy적형모。이PPy위재체,통과흡부법고정포도당양화매（GOD）,득도GOD/PPy/GC전겁。이용순배복안법대GOD/PPy/GC전겁적전화학행위진행분석,결과표명,이PPy위재체가이흔호지고정GOD병보지기생물활성。재0.1mol/L린산염완충용액중,무임하전자매개체존재시,GOD/PPy/GC전겁현시료흔호적전최화성능。
Nano-polypyrrole（PPy） were prepared on glassy carbon（GC） electrodes by electrodeposited method.The morphology of the PPy was obtained by scanning electron microscopy（SEM）.The GOD/PPy/GC electrode was prepared by immobilization glucose oxidase（GOD） on the PPy.The electrocatalytic properties of GOD/PPy/GC electrode were characterized by cyclic voltograme method;the results showed that GOD can be immobilized on the nano-PPy and the biological activation of GOD was retained.The GOD/PPy/GC electrode exhibits good eleccatalytic performance without any electron mediator at the medium of phosphate buffer（0.1mol/L）.