CAJ | 학술논문

为治疗牙本质过敏症,研究了海藻酸钙对牙本质小管的封闭作用及矿化效果。以海藻酸钠（SA）,碳酸钙,葡萄糖酸内酯（GDL）为原料,在牙本质上通过原位相转变制备海藻酸钙（CA）凝胶。测定了单一变量条件下不同海藻酸钠浓度、f值（钙离子与羧基的摩尔比）及n值（葡萄糖酸内酯与钙离子的摩尔比）对海藻酸钙凝胶的凝胶化时间和浸提液pH值的影响。扫描电镜观察海藻酸钠混合液处理前后牙本质微观形貌改变,并对封闭后牙本质小管进行了矿化研究。结果表明海藻酸钠浓度为2.5%、f=0.5及n=0.6为最佳配比。海藻酸钠处理后,开放的牙本质小管明显变小或封闭,显示海藻酸钠溶液可进入牙本质小管形成凝胶,并封闭牙本质小管。矿化后凝胶表面呈致密的矿化层,XRD分析为单一的高结晶羟基磷灰石,CA凝胶有利于诱导高结晶度HA的生成,为继续探索牙本质的仿生矿化提供新思路。
위치료아본질과민증,연구료해조산개대아본질소관적봉폐작용급광화효과。이해조산납（SA）,탄산개,포도당산내지（GDL）위원료,재아본질상통과원위상전변제비해조산개（CA）응효。측정료단일변량조건하불동해조산납농도、f치（개리자여최기적마이비）급n치（포도당산내지여개리자적마이비）대해조산개응효적응효화시간화침제액pH치적영향。소묘전경관찰해조산납혼합액처리전후아본질미관형모개변,병대봉폐후아본질소관진행료광화연구。결과표명해조산납농도위2.5%、f=0.5급n=0.6위최가배비。해조산납처리후,개방적아본질소관명현변소혹봉폐,현시해조산납용액가진입아본질소관형성응효,병봉폐아본질소관。광화후응효표면정치밀적광화층,XRD분석위단일적고결정간기린회석,CA응효유리우유도고결정도HA적생성,위계속탐색아본질적방생광화제공신사로。
This study investigated the role of calcium alginate gel in the occlusion and mineralization of dentinal tubules to treat dentine hypersensitivity.Calcium alginate gel was formed from sodium alginate（SA）,calcium carbonate and gluconolactone（GDL） by in situ phase transition on dentin.The gel formation time and the pH value of the extract were measured with changes in a single variable of calcium alginate concentration,f value（the molar ratio of calcium ion and carboxyl） or n value（the molar ratio of GDL and calcium ion）.The structure and morphology of dentinal tubules were observed by scanning electron microscopy（SEM） before and after the calcium alginate treatment.The results showed that the gel formation was optimized at sodium alginate concentration of 2.5%,f value of 0.5 and at n value of 0.6.The open tubules became narrower or occluded after the calcium alginate treatment.Layers of dense mineral deposits were seen on the surface of the gel in the dentinal tubules.The XRD analysis demonstrated that the mineral deposits were highly crystallized hydroxyapatite（HA） only.Altogether,our results indicate that calcium alginate mixture occludes the dentinal tubules by intratubular gel formation,and that the formed gel induces the deposit of highly crystallized HA providing a new way of further exploring the dentin biomineralization.