CAJ | 학술논문

采用物理化学稳定性较好的石英玻璃为基底，用非平衡磁控溅射技术于其表面沉积生物相容性良好的钛薄膜，然后在钛薄膜表面共价固定纤连蛋白。采用傅立叶红外光谱（FT—IR）和X射线光电子能谱（XPS）对各步处理后的材料表面特征进行检测和分析。主要研究了用MicroBCA检测法对样品表面固定的纤连蛋白的定量表征的可行性，并将该结果与用石英晶体微天平（QCM）方法检测获得的纤连蛋白量做了对比和深入分析。结果显示，纤连蛋白可以成功固定到钛薄膜表面，MicroBCA检测法和QCM法的检测结果具有一致性，样品表面纤连蛋白的固定量大约在767～789ng／cm2。
채용물이화학은정성교호적석영파리위기저，용비평형자공천사기술우기표면침적생물상용성량호적태박막，연후재태박막표면공개고정섬련단백。채용부립협홍외광보（FT—IR）화X사선광전자능보（XPS）대각보처리후적재료표면특정진행검측화분석。주요연구료용MicroBCA검측법대양품표면고정적섬련단백적정량표정적가행성，병장해결과여용석영정체미천평（QCM）방법검측획득적섬련단백량주료대비화심입분석。결과현시，섬련단백가이성공고정도태박막표면，MicroBCA검측법화QCM법적검측결과구유일치성，양품표면섬련단백적고정량대약재767～789ng／cm2。
The aim of this study was to assess the feasibility of MicroBCA method in quantification of fibronectin immobilized on solid material surfaces. In this study, titanium films with favourable physiochemica] perform- ance and biocompatibility were firstly deposited onto quartz glass surfaces via unbalanced magnetron sputtering system. Then, fibronectin was covalently immobilized onto coated titanium film surface. Fourier transform in- frared spectroscopy （FT-IR） and X-ray photoelectron spectroscopy （XPS） were used to determine the chemical composition of the surfaces at various stages of surface modification to verify the protein was successfully immo- bilized. MicroBCA protein assay method was used to determine the amount of fibronectin on sample surfaces. As comparison, the mass of immobilized protein was also detected by quartz crystal microbalance （QCM） tech- nical. The FT-IR and XPS results showed that fibronectin was successfully immobilized onto the coated titani- um film surface. The comparison results from MicroBCA and QCM measurements were consistent tendency. And the surface density of immobilized fibronectin on sample surface was approximately 767-789ng/cm2.