功能材料
功能材料
공능재료
JOURNAL OF FUNCTIONAL MATERIALS
2012年
18期
2473-2477
,共5页
钱宇娜%李林昊%蒋超%吕永钢%钟莉%杨力
錢宇娜%李林昊%蔣超%呂永鋼%鐘莉%楊力
전우나%리림호%장초%려영강%종리%양력
丝素蛋白%透明质酸%纳米纤维%组织工程
絲素蛋白%透明質痠%納米纖維%組織工程
사소단백%투명질산%납미섬유%조직공정
silk fibroin (SF)%hyaluronic acid (HA)%nanofibers%tissue engineering
生物材料组成成分对细胞生物功能有不同的影响。利用静电纺丝技术制备了基于聚己内酯(PCL,polycaprolactone)的不同天然蛋白、多糖(丝素蛋白(SF,silk fibroin)、透明质酸(HA,hyaluronicacid))的混合组分纳米纤维,采用了扫描电镜和接触角对纳米纤维进行基础表征。同时,进一步考察了纳米纤维作为组织工程支架的可行性。研究结果表明SF组分能增加材料的可纺性,有利于细胞的前期黏附,并能够促进细胞增殖。HA组分可以改善材料的亲水性,增加细胞伪足并促进细胞迁移。重要的是,PCL/SF/HA纳米纤维能同时结合SF和HA的优点,有望在组织工程领域得到应用。
生物材料組成成分對細胞生物功能有不同的影響。利用靜電紡絲技術製備瞭基于聚己內酯(PCL,polycaprolactone)的不同天然蛋白、多糖(絲素蛋白(SF,silk fibroin)、透明質痠(HA,hyaluronicacid))的混閤組分納米纖維,採用瞭掃描電鏡和接觸角對納米纖維進行基礎錶徵。同時,進一步攷察瞭納米纖維作為組織工程支架的可行性。研究結果錶明SF組分能增加材料的可紡性,有利于細胞的前期黏附,併能夠促進細胞增殖。HA組分可以改善材料的親水性,增加細胞偽足併促進細胞遷移。重要的是,PCL/SF/HA納米纖維能同時結閤SF和HA的優點,有望在組織工程領域得到應用。
생물재료조성성분대세포생물공능유불동적영향。이용정전방사기술제비료기우취기내지(PCL,polycaprolactone)적불동천연단백、다당(사소단백(SF,silk fibroin)、투명질산(HA,hyaluronicacid))적혼합조분납미섬유,채용료소묘전경화접촉각대납미섬유진행기출표정。동시,진일보고찰료납미섬유작위조직공정지가적가행성。연구결과표명SF조분능증가재료적가방성,유리우세포적전기점부,병능구촉진세포증식。HA조분가이개선재료적친수성,증가세포위족병촉진세포천이。중요적시,PCL/SF/HA납미섬유능동시결합SF화HA적우점,유망재조직공정영역득도응용。
The scaffold compositions played different roles on cellular biological functions.In this study,we developed polycaprolactone(PCL) based nanofibers composed of natural protein and polysaccharides(hyaluronan(HA),silk fibroin(SF)) via electrospinning technology.These nanofibers were characterized by scanning electron microscopy(SEM) and contact angle.Furthermore,we evaluated the potential of different composite nanofibers as tissue engineering scaffolds.In vitro cultivation of human primary skin fibroblasts on the SF-based scaffolds showed a significant increase in cell proliferation.A significant number of cells were found to have well-developed cytoskeleton on PCL/SF nanofibers.Addition of HA component transformed current PCL and PCL/SF components into hydrophilic fibers.Importantly,HA-based scaffolds significantly enhanced cell filopodia protrusions and migration in vitro.PCL/SF/HA nanofibers could combine the advantages of SF and HA.These findings suggest that such multiple blended nanofibers may offer possibilities to tissue engineering application.