CAJ | 학술논문

构建了PNIPAAm-g-EVA/明胶支架复合人工皮肤样品,并初步考察了该人工皮肤的细胞相容性。由于明胶支架的生物相容性已经得到充分验证,主要选择成纤维细胞为实验细胞,PNIPAAm-g-EVA膜为研究对象。采用含有胶原酶的消化液消化组织块,在较短时间内可获得足够数量的成纤维细胞。成纤维细胞与PNIPAAm-g-EVA膜共培养结果表明,成纤维细胞在膜表面粘附力强,生长、增殖情况良好。说明与PNIPAAm-g-EVA膜共培养并未影响细胞的正常生理功能,PNIPAAm-g-EVA具有良好的细胞相容性。
구건료PNIPAAm-g-EVA/명효지가복합인공피부양품,병초보고찰료해인공피부적세포상용성。유우명효지가적생물상용성이경득도충분험증,주요선택성섬유세포위실험세포,PNIPAAm-g-EVA막위연구대상。채용함유효원매적소화액소화조직괴,재교단시간내가획득족구수량적성섬유세포。성섬유세포여PNIPAAm-g-EVA막공배양결과표명,성섬유세포재막표면점부력강,생장、증식정황량호。설명여PNIPAAm-g-EVA막공배양병미영향세포적정상생리공능,PNIPAAm-g-EVA구유량호적세포상용성。
In this study a gelatin/PNIPAAm-g-EVA bilayer artificial skin had been constructed and its cell com- patibility had also been assessed. As the good biocompatibility of gel had already been proved. So we focus on assessing the cell compatibility of PNIPAAm-g-EVA membrane. Rat skin fibroblasts were cultured in vitro with PNIPAAm-g-EVA membrane for different periods, and the results indicated that the proliferation of fibro- blasts on PNIPAAm-g-EVA membrane increased with time significantly. Imnunohistochemical staining of fi- broblasts were positive. Therefore, it can be concluded that the PNIPAAm-g-EVA membrane has good cyto- compatibility on the basis of positive effect on the attachment, proliferation and growth of fibroblasts.