CAJ | 학술논문

支架表面内皮化成为最终实现支架与血液及组织长期相容的良好途径,内皮祖细胞在材料表面的集聚、生长与增殖使快速内皮化成为可能。利用静电结合作用将抗凝血药物肝素和内皮细胞基膜中重要组成的Ⅳ型胶原结合到Ti表面,使其具有良好的细胞相容性。Ti通过碱活化和硅烷化处理后,形成带正电荷的氨基表面,与有残留负电荷的肝素/Ⅳ型胶原复合物静电结合,形成生物化层。通过傅立叶红外掠射检测了材料表面特征基团的变化,通过水接触角的测定和扫描电子显微镜跟踪表面亲疏水性能以及表面形貌的变化。通过免疫荧光染色法对Ⅳ型胶原进行定性表征。结果显示仿生层构建成功,修饰后的表面较Ti表面能够粘附更多的内皮祖细胞,细胞生长与增殖较快,且细胞形态良好,细胞相容性得到明显改善。Ⅳ型胶原的引入对于心血管植入器械的表面改性有重要参考价值。
지가표면내피화성위최종실현지가여혈액급조직장기상용적량호도경,내피조세포재재료표면적집취、생장여증식사쾌속내피화성위가능。이용정전결합작용장항응혈약물간소화내피세포기막중중요조성적Ⅳ형효원결합도Ti표면,사기구유량호적세포상용성。Ti통과감활화화규완화처리후,형성대정전하적안기표면,여유잔류부전하적간소/Ⅳ형효원복합물정전결합,형성생물화층。통과부립협홍외략사검측료재료표면특정기단적변화,통과수접촉각적측정화소묘전자현미경근종표면친소수성능이급표면형모적변화。통과면역형광염색법대Ⅳ형효원진행정성표정。결과현시방생층구건성공,수식후적표면교Ti표면능구점부경다적내피조세포,세포생장여증식교쾌,차세포형태량호,세포상용성득도명현개선。Ⅳ형효원적인입대우심혈관식입기계적표면개성유중요삼고개치。
Endothelialization is a good way to obtain ultimate long-term compatibility of the interfaces between stents and blood or/and tissue.Rapid endothelialization on the biomaterials surface is possible through the aggregation,growth and proliferation of endothelial progenitor cells.In this paper,the anti-clotting drug of heparin and type Ⅳ collagen as a main component of basement membrane were combined onto the titanium surface to improve the biocompatibility.The formation of positively charged amino surface on titanium was firstly completed after alkali activation and silane treatment.The residual negative charges of heparin/type Ⅳ collagen complex were then combined to the positively charged amino surface via electrostatic interaction.The change of the groups on the surface was detected by the Fourier transform infrared spectroscopy.The water contact angle measurement and scanning electron microscopy were used to track the surface hydrophilic and hydrophobic properties and surface morphology changes.Qualitative characterization of type Ⅳ collagen and the morphology of endothelial progenitor cells were detected by immunofluorescence staining.The results showed that the bioinspired layer was successfully constructed.In compared with titanium,the bionic modified surface had more endothelial progenitor cells(EPCs) adhesion,more rapid cell growth and proliferation,and better cell morphology.It was concluded that the cell compatibility was significantly improved due to the biological modification on materials surface.The introductions of type Ⅳ collagen to materials surface provide an important pathway for the surface modification of cardiovascular implants.