CAJ | 학술논문

采用静电层层自组装技术构建了以I型胶原蛋白（COL）为主要构成的纳米超薄膜。该类型薄膜厚度与涂层层数呈线性关系。通过“喷涂”方式，在未经任何理化作用处理的生物可降解聚酯材料表面可原位形成厚度可控的胶原蛋白薄膜结构，改善聚酯材料的亲水性。在小鼠成纤维细胞（L929）培养实验中，聚乳酸（PLA）表面负载多层膜以后，表现出良好的细胞生物相容性，有利于细胞的生长和铺展。
채용정전층층자조장기술구건료이I형효원단백（COL）위주요구성적납미초박막。해류형박막후도여도층층수정선성관계。통과“분도”방식，재미경임하이화작용처리적생물가강해취지재료표면가원위형성후도가공적효원단백박막결구，개선취지재료적친수성。재소서성섬유세포（L929）배양실험중，취유산（PLA）표면부재다층막이후，표현출량호적세포생물상용성，유리우세포적생장화포전。
Nanometer thin films with type I collagen as main component were constructed by electrostatic layer- by-layer self assembly technique. The thickness and the plies of the films show linearly elationship. By the way of spraying, the thickness-controllable collagen films could be in-situ formed on the surface of biodegradable polyester materials without any chemical treatment to improve the hydrophilicity of the polyester materials greatly. In the mouse fibroblast (L929) culture experiments, polylatic acid (PLA) covered multilayer films demonstrated good biocompatibility.