CAJ | 학술논문

目的：探讨聚己内脂－聚乙二醇二嵌段共聚物（ mPEG-PCL）制备的辣椒碱纳米微球的理化性质，通过实验评价其抗胶质瘤效果。方法通过开环聚合法和溶剂分散法制备负载辣椒碱的纳米微球，应用负载荧光素纳米粒子的肿瘤细胞摄取实验以及辣椒碱纳米微球的细胞毒性实验。结果实验显示载药微球具有缓释特性，细胞可通过胞吞作用将纳米微球摄入，负载NIR-797纳米微球可以很好地透过血-脑屏障，辣椒碱纳米微球对U251细胞的生长有明显的抑制作用。结论 mPEG-PCL为载体的辣椒碱纳米微球能够有效抑制胶质瘤细胞增殖，为脑胶质瘤药物治疗提供了一条极有前途的治疗途径。
목적：탐토취기내지－취을이순이감단공취물（ mPEG-PCL）제비적랄초감납미미구적이화성질，통과실험평개기항효질류효과。방법통과개배취합법화용제분산법제비부재랄초감적납미미구，응용부재형광소납미입자적종류세포섭취실험이급랄초감납미미구적세포독성실험。결과실험현시재약미구구유완석특성，세포가통과포탄작용장납미미구섭입，부재NIR-797납미미구가이흔호지투과혈-뇌병장，랄초감납미미구대U251세포적생장유명현적억제작용。결론 mPEG-PCL위재체적랄초감납미미구능구유효억제효질류세포증식，위뇌효질류약물치료제공료일조겁유전도적치료도경。
Objective To explore the physiochemical characteristics of the capsaicin-loaded nanoparticles prepared by methoxy polyethylene glycol-poly ( caprolactone ) ( mPEG-PCL ) amphilic block copolymer, and to evaluate the ability of the capsaicin-loaded nanoparticles to inhibits the human glioblastoma U251 cells growth.Methods mPEG-PCL amphilic block copolymer was synthesized using the ring-opening polymerization method, and the capsaicin-loaded nanoparticles were prepared with the solvent diffusion method.The uptake of nanoparticles in the glioma cells and its ability to inhibit cell proliferation were tested in human glioblastoma U251 cells.Results In vitro drug release assay revealed that the capsaicin-loaded nanoparticles presented slow-release characteristics.The NIR-797 isothiocyanate-loaded nanoparticles was found to cross the blood-brain barrier.In addition, the capsaicin-loaded nanoparticle showed a remarkable inhibition on the growth of U251 cells.Conclusions The capsaicin-loaded nanoparticles can provide an extremely promising approach for chemotherapy of gliomas.