CAJ | 학술논문

目的：观察转录因子YY1（Yin-Yang 1）在垂体腺瘤中的表达情况，并进一步分析沉默YY1对大鼠垂体瘤GH3细胞侵袭性的影响。方法运用免疫组化技术观察YY1在不同类型垂体腺瘤组织中的表达情况。 Western blot实验检测siRNA沉默大鼠垂体瘤GH3细胞中的基因YY1后，YY1蛋白及基质金属蛋白酶MMP-2、MMP-9的表达水平。采用Transwell chamber细胞侵袭实验观察沉默YY1对GH3细胞侵袭的影响。结果 YY1在侵袭性垂体腺瘤中表达升高；沉默YY1后，GH3细胞侵袭能力下降35．7％（P＜0．05）， MMP-2、MMP-9蛋白表达量下降（P＜0．05）。结论转录因子YY1在侵袭性垂体腺瘤中的表达升高，并通过上调MMP-2、MMP-9的表达来促进GH3细胞侵袭性。
목적：관찰전록인자YY1（Yin-Yang 1）재수체선류중적표체정황，병진일보분석침묵YY1대대서수체류GH3세포침습성적영향。방법운용면역조화기술관찰YY1재불동류형수체선류조직중적표체정황。 Western blot실험검측siRNA침묵대서수체류GH3세포중적기인YY1후，YY1단백급기질금속단백매MMP-2、MMP-9적표체수평。채용Transwell chamber세포침습실험관찰침묵YY1대GH3세포침습적영향。결과 YY1재침습성수체선류중표체승고；침묵YY1후，GH3세포침습능력하강35．7％（P＜0．05）， MMP-2、MMP-9단백표체량하강（P＜0．05）。결론전록인자YY1재침습성수체선류중적표체승고，병통과상조MMP-2、MMP-9적표체래촉진GH3세포침습성。
Objective To investigate the expression of transcription factors YY1( Yin-Yang 1) in pituitary adenomas, and further analyse the effect of silencing YY1 on the invasiveness of rat pituitary tumor GH3 cells.Methods Using immunohistochemical technique, we observed the expression of YY1 in various type pituitary adenomas. The expression levels of YY1 protein and Matrix metalloproteinases MMP-2 and MMP-9 protein in rat pituitary tumor GH3 cells transfected with siRNA was measured by western blot .The invasion ability of GH3 cells after silencing YY1 by transwell chamber invasion assay.Results YY1 was increased in invasive pituitary adenomas.By silencing YY1, we found the invasion capability of GH3 cells were reduced by 35.7% (P<0.05), and the expression levels of MMP-2, MMP-9 were reduced(all P<0.05).Conclusion The expression of transcription factors YY1 is increased in invasive pituitary adenomas, which can promote the invasion of rat pituitary tumor GH3 cells by up-regulating the expression of MMP-2 and MMP-9.