CAJ | 학술논문

采用 SSH 技术分析了 VA 菌根处理后福鼎大白茶根系基因差异表达情况，获得了差异序列，序列比对显示，在上调表达序列中包含了3-羟基-3-甲戊二酸单酰辅酶 A还原酶（3-hydroxy-3-methylglutaryl coenzyme A reductase，HMGR）。采用 RACE 技术获得了 HGMR 基因全长序列，HGMR 基因长2420 bp，具有1773 bp开放阅读框（163rd~1935th），编码590个氨基酸。分子生物信息学分析表明，HGMR蛋白分子量约63.5 kD，等电点为6.8，定位于线粒体膜或者内质网膜。研究还显示，HGMR在茶树叶片中表达强度存在明显的品种差异，同时，HGMR对生物性和非生物性胁迫均有明显响应。
채용 SSH 기술분석료 VA 균근처리후복정대백다근계기인차이표체정황，획득료차이서렬，서렬비대현시，재상조표체서렬중포함료3-간기-3-갑무이산단선보매 A환원매（3-hydroxy-3-methylglutaryl coenzyme A reductase，HMGR）。채용 RACE 기술획득료 HGMR 기인전장서렬，HGMR 기인장2420 bp，구유1773 bp개방열독광（163rd~1935th），편마590개안기산。분자생물신식학분석표명，HGMR단백분자량약63.5 kD，등전점위6.8，정위우선립체막혹자내질망막。연구환현시，HGMR재다수협편중표체강도존재명현적품충차이，동시，HGMR대생물성화비생물성협박균유명현향응。
By using SSH, the differences in gene expression of root system from Fuding Dabai tea infected by VA mycorrhiza were analyzed and diversity sequences were obtained. The sequence alignment showed that the mentioned sequence contain 3-hydroxy-3-methylglutaryl coenzyme A reductase named HMGR. HGMR full-length sequence was obtained by using RACE. The length of HGMR gene is 2 420 bp, with 1 773 bp ORF (163rd-1935th), and the sequence encoded 590 amino acids. Bioinformatics indicated that the HGMR protein ’s molecular weight is about 63.5 kD, IEP is 6.8, which located in mitochondria or endoplasmic reticulum membrane. The stud y also showed expression degree of HGMR is distinct in different cultivars, while it responded obviously to biological and non-biological stress.