CAJ | 학술논문

目的：研究P物质（SP）和神经激肽受体-1（NK1R）在乳腺癌中的表达，探讨其改变与乳腺癌患者临床病理特征的相关性。方法采用ELISA法检测乳腺癌及乳腺良性疾病患者血清、乳腺细胞系培养上清中SP水平，用免疫组织化学和免疫印迹技术检测总NK1R、全长型NK1R（ NK1R-FL）和截短型NK1R（ NK1R-Tr）在82例乳腺癌及其癌旁正常组织、30例乳腺良性病变组织中的表达。结果乳腺癌患者血清SP水平高于乳腺良性疾病患者和正常对照（ P＜0．05），乳腺癌组织的NK1R-FL表达显著低于、NK1R-Tr的表达显著高于癌旁正常乳腺组织和乳腺良性病变组织（P＜0．01）；与导管内癌组织比较，NK1R-FL的表达在浸润性导管癌中下降显著（P＜0．05），而NK1R-Tr的表达则明显升高；NK1R-FL表达随TNM分期的升高而降低，而NK1R-Tr和SP的表达则升高（P＜0．01）；乳腺癌患者组织NK1R-FL的表达降低、NK1R-Tr升高和血清的SP升高还与淋巴结转移、雌激素受体（ estro-gen receptor， ER）和孕激素受体（ progesterone receptor， PR）阴性、Ki-67阳性、人表皮生长因子受体-2（human epidermal growth factor receptor 2，HER2）阳性、血管内皮生长因子（vascular endothelial growth factor，VEGF）阳性、血清癌胚抗原（carcino-embryonic antigen，CEA）和糖类抗原-153（cancer antigen 153，CA-153）水平等显著相关（P＜0．05）。乳腺癌组织NK1R-FL和NK1R-Tr的表达与患者的年龄、组织分级和P53表达等临床病理因素均无关（P＞0．05）。结论 NK1R-FL的表达与乳腺癌的浸润转移呈负相关，SP、NK1R-Tr的表达上调与乳腺癌的浸润转移成正相关。
목적：연구P물질（SP）화신경격태수체-1（NK1R）재유선암중적표체，탐토기개변여유선암환자림상병리특정적상관성。방법채용ELISA법검측유선암급유선량성질병환자혈청、유선세포계배양상청중SP수평，용면역조직화학화면역인적기술검측총NK1R、전장형NK1R（ NK1R-FL）화절단형NK1R（ NK1R-Tr）재82례유선암급기암방정상조직、30례유선량성병변조직중적표체。결과유선암환자혈청SP수평고우유선량성질병환자화정상대조（ P＜0．05），유선암조직적NK1R-FL표체현저저우、NK1R-Tr적표체현저고우암방정상유선조직화유선량성병변조직（P＜0．01）；여도관내암조직비교，NK1R-FL적표체재침윤성도관암중하강현저（P＜0．05），이NK1R-Tr적표체칙명현승고；NK1R-FL표체수TNM분기적승고이강저，이NK1R-Tr화SP적표체칙승고（P＜0．01）；유선암환자조직NK1R-FL적표체강저、NK1R-Tr승고화혈청적SP승고환여림파결전이、자격소수체（ estro-gen receptor， ER）화잉격소수체（ progesterone receptor， PR）음성、Ki-67양성、인표피생장인자수체-2（human epidermal growth factor receptor 2，HER2）양성、혈관내피생장인자（vascular endothelial growth factor，VEGF）양성、혈청암배항원（carcino-embryonic antigen，CEA）화당류항원-153（cancer antigen 153，CA-153）수평등현저상관（P＜0．05）。유선암조직NK1R-FL화NK1R-Tr적표체여환자적년령、조직분급화P53표체등림상병리인소균무관（P＞0．05）。결론 NK1R-FL적표체여유선암적침윤전이정부상관，SP、NK1R-Tr적표체상조여유선암적침윤전이성정상관。
Objective To investigate the expression of substance P ( SP) and neurokinin-1 recep-tor (NK1R) in patients with breast cancer and to further understand the correlations of them with the clinico-pathological features and the prognosis of breast cancer.Methods SP levels in serum samples and superna-tants of breast cell culture were measured by ELISA.The expression of total NK1R, full-length NK1R (NK1R-FL) and truncted NK1R (NK1R-Tr) in 82 patients with breast cancer and 30 patients with breast hyperplasia were detected by using immunohistochemistry and Western blot.Results The levels of SP in patients with breast cancer were higher than those in patients with breast hyperplasia and healthy subjects ( P<0.05) .The expression of NK1R-FL was inhibited, while the expression of NK1R-Tr was significantly en-hanced in breast cancer tissues as compared with those in normal and hyperplastic breast tissues (P<0.01). Compared with patients with ductal carcinoma in situ ( DCIS ) , patients with invasive ductal carcinoma (IDC) showed a decreased expression of NK1R-FL, but an increased expression of NK1R-Tr (P<0.05). The stages of breast cancer classified by using the TNM system showed a negative correlation with NK1R-FL expression, but positive correlations with the expression of NK1R-Tr and SP (P<0.01).Moreover, the en-hanced expression of NK1R-Tr and SP and suppressed expression of NK1R-FL were positively related to lymph node metastasis and high expression of vascular endothelial growth factor ( VEGF) , human epidermal growth factor receptor 2 ( HER2) and Ki-67, but negatively related to the expression of estrogen receptor (ER) and progesterone receptor (PR) in breast cancer tissues (P<0.05).The expression of NK1R-FL and NK1R-Tr showed no significant correlation with age, histological grades and the expression of P53 in breast cancer tissues (P>0.05).Conclusion The invasion and metastasis of breast cancer showed a negative cor-relation with the expression of NK1R-FL, but a positive correlation with the expression of NK1R-Tr and SP.