CAJ | 학술논문

目的：应用高分辨率熔解曲线法快速鉴定功能性第2类整合子。方法收集99株2011年8月—2012年8月非重复分离自临床标本的第2类整合酶阳性变形杆菌，以基因组DNA为模板，PCR扩增包含第2类整合酶基因突变位点一段60 bp的片段，高分辨率熔解曲线分析后测序验证。结果功能性第2类整合子与普通第2类整合子高分辨熔解曲线具有显著差别，测序结果与高分辨率熔解曲线法鉴定结果一致。结论应用高分辨率熔解曲线法可快速准确进行功能性第2类整合子的筛查鉴定。
목적：응용고분변솔용해곡선법쾌속감정공능성제2류정합자。방법수집99주2011년8월—2012년8월비중복분리자림상표본적제2류정합매양성변형간균，이기인조DNA위모판，PCR확증포함제2류정합매기인돌변위점일단60 bp적편단，고분변솔용해곡선분석후측서험증。결과공능성제2류정합자여보통제2류정합자고분변용해곡선구유현저차별，측서결과여고분변솔용해곡선법감정결과일치。결론응용고분변솔용해곡선법가쾌속준학진행공능성제2류정합자적사사감정。
Objective To establish a high resolution melting based method for the rapid identifica-tion of functional class 2 integron.Methods Nighty-nine non-repetitive Proteus spp.strains positive for genes encoding class 2 integrase were isolated from August, 2011 to August, 2012.The genomic DNAs were extracted and used as templates to amplify 60 base pair fragments containing the mutated point in class 2 in-tegrase gene by PCR.The high resolution melting analysis was conducted to identify the functional class 2 in-tegrons that were further compared by sequence analysis.Results There were remarkable differences with the high resolution melting curves between the functional class 2 integrons and the ordinary class 2 integrons. The results of high resolution melting analysis were consistent with those by using sequence analysis.Con-clusion High resolution melting analysis could be used for the rapid and accurate identification of functional class 2 integron.