CAJ | 학술논문

目的：探讨外源性白细胞介素-10（ IL-10）对变应性鼻炎大鼠（ AR） Th细胞和Th17细胞相关因子表达的影响。方法 SD大鼠30只，采用随机数字表法将其分为AR模型组、IL-10组和阴性对照组，每组10只。 AR组和IL-10组采用常规卵白蛋白（ OVA）联合氢氧化铝致敏法建立AR大鼠模型，阴性对照组采用生理盐水代替，IL-10组于激发21 d后，给予OVA的同时，腹腔注射40 pg／kg的IL-101 ml，连续7 d；各组于给药后30 min内观察各组大鼠临床表现，记录大鼠的行为学得分，并于最后一次激发后48 h，取血清检测IgE和OVA-sIgE含量，同时取大鼠双侧鼻腔呼吸区黏膜，用ELISA试剂盒检测IFN-γ、IL-5、IL-17和TGF-β含量变化，同时用Western blot法检测各指标的表达水平。结果 AR组大鼠均表现出四处摩擦、涕流满面等重度AR的临床表现，与其相比，IL-10组表现明显减轻（P＜0．05）；各组血清中IgE和OVA-sIgE含量及鼻黏膜中IFN-γ、IL-5、IL-17和TGF-β含量均明显升高（P＜0．05），经IL-10干预后，其含量明显降低，且与AR组相比，差异有统计学意义（P＜0．05）；Western blot结果显示，与对照组相比，AR组和IL-10组大鼠鼻黏膜中IFN-γ、IL-5、IL-17和TGF-β表达水平明显升高，IL-10组大鼠鼻黏膜中IFN-γ、IL-5、IL-17和TGF-β表达水平低于AR组（ P＜0．05）。结论用外源性IL-10的方法治疗AR，并降低鼻黏膜中IFN-γ、IL-5、IL-17和TGF-β的表达，可在一定程度上达到治疗AR的目的。
목적：탐토외원성백세포개소-10（ IL-10）대변응성비염대서（ AR） Th세포화Th17세포상관인자표체적영향。방법 SD대서30지，채용수궤수자표법장기분위AR모형조、IL-10조화음성대조조，매조10지。 AR조화IL-10조채용상규란백단백（ OVA）연합경양화려치민법건립AR대서모형，음성대조조채용생리염수대체，IL-10조우격발21 d후，급여OVA적동시，복강주사40 pg／kg적IL-101 ml，련속7 d；각조우급약후30 min내관찰각조대서림상표현，기록대서적행위학득분，병우최후일차격발후48 h，취혈청검측IgE화OVA-sIgE함량，동시취대서쌍측비강호흡구점막，용ELISA시제합검측IFN-γ、IL-5、IL-17화TGF-β함량변화，동시용Western blot법검측각지표적표체수평。결과 AR조대서균표현출사처마찰、체류만면등중도AR적림상표현，여기상비，IL-10조표현명현감경（P＜0．05）；각조혈청중IgE화OVA-sIgE함량급비점막중IFN-γ、IL-5、IL-17화TGF-β함량균명현승고（P＜0．05），경IL-10간예후，기함량명현강저，차여AR조상비，차이유통계학의의（P＜0．05）；Western blot결과현시，여대조조상비，AR조화IL-10조대서비점막중IFN-γ、IL-5、IL-17화TGF-β표체수평명현승고，IL-10조대서비점막중IFN-γ、IL-5、IL-17화TGF-β표체수평저우AR조（ P＜0．05）。결론용외원성IL-10적방법치료AR，병강저비점막중IFN-γ、IL-5、IL-17화TGF-β적표체，가재일정정도상체도치료AR적목적。
Objective To investigate the expression of IFN-γ, IL-5, IL-17 and TGF-βby Th cells and Th17 cells in rats with allergic rhinitis upon the intervention of IL-10.Methods SD rats were ran-domly divided into three groups including allergic rhinitis ( AR ) group, IL-10 treated group and control group (n=10).Rats in AR group and IL-10 treated group were sensitized by injection of ovalbumin (OVA) and aluminum hydroxide on the 1st, the 7th and the 14th days.The rats treated with equal volume of saline were set up as the control.The corresponding interventions ( OVA, OVA and IL-10, saline) were respec-tively given to rats in each group on the 21th day for 7 consecutive days.The clinical manifestations in rats were observed within 30 minutes after each administration.Serum samples were collected at 48 hours after the last challenge for the detection of IgE and OVA-sIgE.ELISA and Western blot assay were performed to detect IFN-γ, IL-5, IL-17 and TGF-βin nasal mucosa samples.Results Some characteristic symptoms of AR were observed in rats from AR group and IL-10 treated group.Compared with IL-10 treated rats, rats in AR group showed severe clinical symptoms such as constant rubbing and tearing of the eyes (P<0.05).The levels of IgE and OVA-sIgE in serum samples and the levels of IFN-γ, IL-5, IL-17 and TGF-βin nasal tis-sues were significantly increased in rats with RA (P<0.05), but were reduced with IL-10 intervention (P<0.05).Conclusion Exogenous IL-10 could be used to treat AR by reducing the expression of IFN-γ, IL-5, IL-17 and TGF-βin nasal tissues.