CAJ | 학술논문

目的：探讨芍药苷（ PF）对乙醇诱导L02细胞细胞色素P450（ CYP450）2E1表达及脂质过氧化的影响。方法用75 mmol· L－1乙醇体外诱导培养法建立L02细胞损伤模型，检测细胞培养液上清超氧化物歧化酶（ SOD ）和丙二醛（ MDA）含量的变化，用定时定量－聚合酶链反应（ RT－PCR）及细胞免疫化学技术观察芍药苷处理后的乙醇损伤L02细胞中CYP4502E1的表达变化。结果与对照组比较，模型组血清SOD显著降低，MDA增高（P＜0.05），CYP4502E1 mRNA及其蛋白表达明显增强（ P＜0.05）；与模型组相比，芍药苷组SOD增高， MDA降低，CYP4502E1 mRNA及其蛋白表达水平下降（P＜0.05）。结论芍药苷对L02细胞乙醇损伤具有明显的改善作用，减轻脂质过氧化，其作用可能与调节CYP4502E1的表达有关。
목적：탐토작약감（ PF）대을순유도L02세포세포색소P450（ CYP450）2E1표체급지질과양화적영향。방법용75 mmol· L－1을순체외유도배양법건립L02세포손상모형，검측세포배양액상청초양화물기화매（ SOD ）화병이철（ MDA）함량적변화，용정시정량－취합매련반응（ RT－PCR）급세포면역화학기술관찰작약감처리후적을순손상L02세포중CYP4502E1적표체변화。결과여대조조비교，모형조혈청SOD현저강저，MDA증고（P＜0.05），CYP4502E1 mRNA급기단백표체명현증강（ P＜0.05）；여모형조상비，작약감조SOD증고， MDA강저，CYP4502E1 mRNA급기단백표체수평하강（P＜0.05）。결론작약감대L02세포을순손상구유명현적개선작용，감경지질과양화，기작용가능여조절CYP4502E1적표체유관。
Objective To investigate the effect of paeoniflorin ( PF) on cytochrome P450 2E1( CYP450 2E1) and lipid peroxidation in L02 cell induced by ethanol.Methods L02 cell model was established by 75 mmol · L-1 ethanol.After treated with paeoniflorin, superoxide dismutase ( SOD) and malondialdehyde ( MDA) levels in the cell culture supernatant were detected by biochemical analysis.The expressions of CYP450 2E1 in L02 cell were observed by RT-PCR and immunocyto-chemistry.Results Compared with the control group, serum SOD levels in model group were significantly decreased, while MDA levels were elevated ( P <0.05 ).The levels of CYP450 2E1 mRNA and protein were significantly increased in model group (P<0.05).However, com-pared with the model group, SOD levels in the cell culture supernatant of PF group were much higher, while MDA levels were decreased, and the levels of CYP450 2E1 mRNA and protein were significantly decreased in PF group ( P<0.05 ).Conclusion The effect of PF on the improve-ment of L02 cell damaged by ethanol and reducing lipid peroxidation could be related with the regulation on the expression of CYP450 2 E1.